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https://hdl.handle.net/2440/55115
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Type: | Journal article |
Title: | Cytotoxicity screening for the cyanobacterial Toxin cylindrospermopsin |
Author: | Froscio, S. Fanok, S. Humpage, A. |
Citation: | Journal of Toxicology and Environmental Health Part A: Current Issues, 2009; 72(5):345-349 |
Publisher: | Taylor & Francis Ltd |
Issue Date: | 2009 |
ISSN: | 1528-7394 1087-2620 |
Statement of Responsibility: | Suzanne M. Froscio, Stella Fanok and Andrew R. Humpage |
Abstract: | The cell lines C3A, HepG2, NCI-87, HCT-8, HuTu-80, Caco-2, and Vero were screened for sensitivity to the cyanobacterial toxin cylindrospermopsin (CYN), with the aim of determining the most sensitive cells to be used in cytotoxicity tests. Cell lines were chosen to be representative of the organs targeted by the toxin; liver, kidney, intestine, and were expected to have different metabolic activities and uptake capabilities. Over the range of cell lines tested, IC₅₀ determinations at 24 h (MTT assay) ranged fourfold, from 1.5 μM for hepatocyte-derived cell lines (C3A IC₅₀ = 1.5 ± 0.54; HepG2 IC₅₀ = 1.5 ± 0.87) to 6.5 ± 3.3μM for the colon-derived Caco-2 cell line. The cell-line sensitivity seemed to decrease in cell lines derived from progressively more distal regions of the gastrointestinal tract: gastric > duodenal > ileal > colonic. The greater sensitivity of the hepatic cell lines to CYN was also apparent in 7-d exposure studies, with low toxin concentrations exerting cytotoxic effects that were not seen in other cell lines. Short-term exposure of C3A cells to CYN (1-6 h) was shown to induce cytotoxicity at 24 h despite a washout and recovery incubation, demonstrating the protracted and apparently irreversible nature of CYN's toxic effects. |
Keywords: | Cell Line Animals Humans Cyanobacteria Alkaloids Tetrazolium Salts Uracil Thiazoles Bacterial Toxins Cell Survival Time Factors Cyanobacteria Toxins |
DOI: | 10.1080/15287390802529906 |
Published version: | http://dx.doi.org/10.1080/15287390802529906 |
Appears in Collections: | Aurora harvest 5 Environment Institute publications Pharmacology publications |
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