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https://hdl.handle.net/2440/5541
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Type: | Journal article |
Title: | Endotoxin-induced inflammation does not cause hepatic zinc accumulation in mice lacking metalluthionein gene expression |
Author: | Philcox, J. Coyle, P. Michalska, A. Choo, C. Rofe, A. |
Citation: | Biochemical Journal, 1995; 308(2):543-546 |
Publisher: | Portland Press Ltd. |
Issue Date: | 1995 |
ISSN: | 0264-6021 1470-8728 |
Abstract: | The action of endotoxin lipopolysaccharide (LPS) on hepatic Zn uptake was examined in mice lacking expression of metallothionein (MT)-1 and MT-II genes. Hepatic Zn concentrations, which in normal control mice increased by a mean 29% (MT elevated 20-fold) 16 h post-LPS exposure, did not increase in MT-null mice. Plasma Zn fell by 68% in controls and 32% in MT-null mice. The time course of LPS action in normal mice was characterized by a rapid reduction (-74% at 4 h, -81% at 8 h) and partial recovery (-39% at 24 h) in plasma Zn, with a progressive increase over 24 h in hepatic concentrations of MT (by 36-fold) and Zn (by 40%). In contrast, the MT-null mice had a linear decrease in plasma Zn (-15% at 8 h, -41% at 24 h) and early loss of Zn from the liver. The Zn changes seen in MT-null mice were largely attributable to LPS-associated anorexia. Food deprivation (20 h) alone caused respective 14% and 30% decreases in hepatic and plasma Zn concentrations and a 27% reduction in total liver Zn reserves, whereas fasted normal mice conserved Zn with a 4-fold increase in hepatic MT. This study confirms that MT synthesis is essential for endotoxin-induced liver Zn accumulation. |
Keywords: | Liver Animals Mice, Inbred C57BL Mice, Knockout Mice Escherichia coli Inflammation Zinc Lipopolysaccharides Metallothionein Food Deprivation Male |
DOI: | 10.1042/bj3080543 |
Published version: | http://dx.doi.org/10.1042/bj3080543 |
Appears in Collections: | Aurora harvest Pathology publications |
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