Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/5541
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Type: Journal article
Title: Endotoxin-induced inflammation does not cause hepatic zinc accumulation in mice lacking metalluthionein gene expression
Author: Philcox, J.
Coyle, P.
Michalska, A.
Choo, C.
Rofe, A.
Citation: Biochemical Journal, 1995; 308(2):543-546
Publisher: Portland Press Ltd.
Issue Date: 1995
ISSN: 0264-6021
1470-8728
Abstract: The action of endotoxin lipopolysaccharide (LPS) on hepatic Zn uptake was examined in mice lacking expression of metallothionein (MT)-1 and MT-II genes. Hepatic Zn concentrations, which in normal control mice increased by a mean 29% (MT elevated 20-fold) 16 h post-LPS exposure, did not increase in MT-null mice. Plasma Zn fell by 68% in controls and 32% in MT-null mice. The time course of LPS action in normal mice was characterized by a rapid reduction (-74% at 4 h, -81% at 8 h) and partial recovery (-39% at 24 h) in plasma Zn, with a progressive increase over 24 h in hepatic concentrations of MT (by 36-fold) and Zn (by 40%). In contrast, the MT-null mice had a linear decrease in plasma Zn (-15% at 8 h, -41% at 24 h) and early loss of Zn from the liver. The Zn changes seen in MT-null mice were largely attributable to LPS-associated anorexia. Food deprivation (20 h) alone caused respective 14% and 30% decreases in hepatic and plasma Zn concentrations and a 27% reduction in total liver Zn reserves, whereas fasted normal mice conserved Zn with a 4-fold increase in hepatic MT. This study confirms that MT synthesis is essential for endotoxin-induced liver Zn accumulation.
Keywords: Liver; Animals; Mice, Inbred C57BL; Mice, Knockout; Mice; Escherichia coli; Inflammation; Zinc; Lipopolysaccharides; Metallothionein; Food Deprivation; Male
RMID: 0030006204
DOI: 10.1042/bj3080543
Appears in Collections:Pathology publications

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