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https://hdl.handle.net/2440/55844
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dc.contributor.author | Kidd, S. | - |
dc.contributor.author | Jiang, D. | - |
dc.contributor.author | Jennings, M. | - |
dc.contributor.author | McEwan, A. | - |
dc.date.issued | 2007 | - |
dc.identifier.citation | Infection and Immunity, 2007; 75(9):4506-4513 | - |
dc.identifier.issn | 0019-9567 | - |
dc.identifier.issn | 1098-5522 | - |
dc.identifier.uri | http://hdl.handle.net/2440/55844 | - |
dc.description.abstract | In Haemophilus influenzae Rd KW20, we identified a gene, adhC, which encodes a class III alcohol dehydrogenase (AdhC) and has S-nitrosoglutathione reductase activity. adhC exists on an operon with estD, which encodes an esterase. Divergent to the adhC-estD operon is the Haemophilus influenzae nmlR gene (nmlR(HI)), which encodes a MerR family regulator that is homologous to the Neisseria MerR-like regulator (NmlR). Analysis of an nmlR(HI) mutant indicated that expression of the adhC-estD operon is regulated by NmlR(HI) in strain Rd KW20. Chromosomal inactivation of either adhC or nmlR(HI) resulted in sensitivity to S-nitrosoglutathione and decreased S-nitrosoglutathione reductase activity. Examination of the NmlR(HI)-AdhC system in the genome sequences of nontypeable H. influenzae strains R2846, R2866, and 86-028NP identified significant variations. The adhC gene of 86-028NP was predicted to be nonfunctional due to a premature stop codon. Polymorphisms in the operator/promoter region of R2866 resulted in reduced enzyme activity. This correlated with an increased sensitivity to S-nitrosoglutathione. The adhC-nmlR(HI) system was examined in thirty-three clinical isolates (both capsular and nontypeable strains). Nucleic acid sequence data showed that only strain 86-028NP contained a premature stop codon. There were some variations in the DNA sequence of the operator/promoter region which altered the nmlR(HI) promoter. However, the clinical isolates still possessed S-nitrosoglutathione reductase activity and showed at least the equivalent ability to grow in the presence of S-nitrosoglutathione as Rd KW20. These data suggest that the nmlR(HI)-adhC system has a role in the defense against nitrosative stress in Haemophilus influenzae. | - |
dc.description.statementofresponsibility | Kidd, SP; Jiang, D; Jennings, MP. and McEwan, AG. | - |
dc.description.uri | http://www.ncbi.nlm.nih.gov/sites/entrez | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Microbiology | - |
dc.source.uri | http://dx.doi.org/10.1128/iai.00487-07 | - |
dc.subject | Haemophilus influenzae | - |
dc.subject | Reactive Nitrogen Species | - |
dc.subject | S-Nitrosoglutathione | - |
dc.subject | Aldehyde Oxidoreductases | - |
dc.subject | Transcription Factors | - |
dc.subject | Gene Expression Regulation, Bacterial | - |
dc.subject | Base Sequence | - |
dc.subject | Oxidative Stress | - |
dc.subject | Molecular Sequence Data | - |
dc.title | Glutathione-dependent alcohol dehydrogenase AdhC is required for defense against nitrosative stress in Haemophilus influenzae. | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/IAI.00487-07 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Kidd, S. [0000-0002-2118-1651] | - |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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