Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Lactoferrin is a potent regulator of bone cell activity and increases bone formation in vivo|
|Citation:||Endocrinology, 2004; 145(9):4366-4374|
|Jillian Cornish, Karen E. Callon, Dorit Naot, Kate P. Palmano, Tatjana Banovic, Usha Bava, Maureen Watson, Jian-Ming Lin, P. C. Tong, Qi Chen, Vincent A. Chan, Helen E. Reid, Nick Fazzalari, Heather M. Baker, Edward N. Baker, Neill W. Haggarty, Andrew B. Grey and Ian R. Reid|
|Abstract:||Lactoferrin is an iron-binding glycoprotein present in epithelial secretions, such as milk, and in the secondary granules of neutrophils. We found it to be present in fractions of milk protein that stimulated osteoblast growth, so we assessed its effects on bone cell function. Lactoferrin produced large, dose-related increases in thymidine incorporation in primary or cell line cultures of human or rat osteoblast-like cells, at physiological concentrations (1-100 microg/ml). Maximal stimulation was 5-fold above control. Lactoferrin also increased osteoblast differentiation and reduced osteoblast apoptosis by up to 50-70%. Similarly, lactoferrin stimulated proliferation of primary chondrocytes. Purified, recombinant, human, or bovine lactoferrins had similar potencies. In mouse bone marrow cultures, osteoclastogenesis was dose-dependently decreased and was completely arrested by lactoferrin, 100 microg/ml, associated with decreased expression of receptor activator of nuclear factor-kappaB ligand. In contrast, lactoferrin had no effect on bone resorption by isolated mature osteoclasts. Lactoferrin was administered over calvariae of adult mice for 5 d. New bone formation, assessed using fluorochrome labels, was increased 4-fold by a 4-mg dose of lactoferrin. Thus, lactoferrin has powerful anabolic, differentiating, and antiapoptotic effects on osteoblasts and inhibits osteoclastogenesis. Lactoferrin is a potential therapeutic target in bone disorders such as osteoporosis and is possibly an important physiological regulator of bone growth.|
Bone Marrow Cells
Organ Culture Techniques
|Rights:||Copyright © 2004 by The Endocrine Society|
|Appears in Collections:||Aurora harvest 5|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.