Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/5613
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Type: Journal article
Title: Lactoferrin is a potent regulator of bone cell activity and increases bone formation in vivo
Author: Cornish, J.
Callon, K.
Naot, D.
Palmano, K.
Banovic, T.
Bava, U.
Watson, M.
Lin, J.
Tong, P.
Chen, Q.
Chan, V.
Reid, H.
Fazzalari, N.
Baker, H.
Baker, E.
Haggarty, N.
Grey, A.
Reid, I.
Citation: Endocrinology, 2004; 145(9):4366-4374
Publisher: Endocrine Soc
Issue Date: 2004
ISSN: 0013-7227
1945-7170
Statement of
Responsibility: 
Jillian Cornish, Karen E. Callon, Dorit Naot, Kate P. Palmano, Tatjana Banovic, Usha Bava, Maureen Watson, Jian-Ming Lin, P. C. Tong, Qi Chen, Vincent A. Chan, Helen E. Reid, Nick Fazzalari, Heather M. Baker, Edward N. Baker, Neill W. Haggarty, Andrew B. Grey and Ian R. Reid
Abstract: Lactoferrin is an iron-binding glycoprotein present in epithelial secretions, such as milk, and in the secondary granules of neutrophils. We found it to be present in fractions of milk protein that stimulated osteoblast growth, so we assessed its effects on bone cell function. Lactoferrin produced large, dose-related increases in thymidine incorporation in primary or cell line cultures of human or rat osteoblast-like cells, at physiological concentrations (1-100 microg/ml). Maximal stimulation was 5-fold above control. Lactoferrin also increased osteoblast differentiation and reduced osteoblast apoptosis by up to 50-70%. Similarly, lactoferrin stimulated proliferation of primary chondrocytes. Purified, recombinant, human, or bovine lactoferrins had similar potencies. In mouse bone marrow cultures, osteoclastogenesis was dose-dependently decreased and was completely arrested by lactoferrin, 100 microg/ml, associated with decreased expression of receptor activator of nuclear factor-kappaB ligand. In contrast, lactoferrin had no effect on bone resorption by isolated mature osteoclasts. Lactoferrin was administered over calvariae of adult mice for 5 d. New bone formation, assessed using fluorochrome labels, was increased 4-fold by a 4-mg dose of lactoferrin. Thus, lactoferrin has powerful anabolic, differentiating, and antiapoptotic effects on osteoblasts and inhibits osteoclastogenesis. Lactoferrin is a potential therapeutic target in bone disorders such as osteoporosis and is possibly an important physiological regulator of bone growth.
Keywords: Cartilage
Skull
Kidney
Bone Marrow Cells
Chondrocytes
Osteoclasts
Osteoblasts
Milk
Milk, Human
Animals
Cattle
Humans
Mice
Rats
Lactoferrin
Organ Culture Techniques
Cell Division
Apoptosis
Cell Differentiation
Cricetinae
Male
Rights: Copyright © 2004 by The Endocrine Society
DOI: 10.1210/en.2003-1307
Appears in Collections:Aurora harvest 5
Pathology publications

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