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https://hdl.handle.net/2440/56669
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dc.contributor.author | Van Deun, A. | - |
dc.contributor.author | Barrera, L. | - |
dc.contributor.author | Bastian, I. | - |
dc.contributor.author | Fattorini, L. | - |
dc.contributor.author | Hoffmann, H. | - |
dc.contributor.author | Kam, K. | - |
dc.contributor.author | Rigouts, L. | - |
dc.contributor.author | Rusch-Gerdes, S. | - |
dc.contributor.author | Wright, A. | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Journal of Clinical Microbiology, 2009; 47(11):3501-3506 | - |
dc.identifier.issn | 0095-1137 | - |
dc.identifier.issn | 1098-660X | - |
dc.identifier.uri | http://hdl.handle.net/2440/56669 | - |
dc.description | Copyright © 2009, American Society for Microbiology. All Rights Reserved. | - |
dc.description.abstract | The objectives of this study were to investigate the origin of highly discordant rifampin (rifampicin) (RMP) drug susceptibility test results obtained for Mycobacterium tuberculosis strains during proficiency testing. Nine Supra-National Tuberculosis Reference Laboratories tested the RMP susceptibilities of 19 selected M. tuberculosis strains, using standard culture-based methods. The strains were classified as definitely resistant (R) (n = 6) or susceptible (S) (n = 2) or probably resistant (PR) (n = 8) or susceptible (PS) (n = 3) based on rpoB mutations and treatment outcome. All methods yielded a susceptible result for the two S and three PS strains lacking an rpoB mutation and a resistant result for one R strain with a Ser531Leu mutation and one PR strain with a double mutation. Although the remaining 12 R and PR strains had rpoB mutations (four Asp516Tyr, three Leu511Pro, two Leu533Pro, one each His526Leu/Ser, and one Ile572Phe), they were all susceptible by the radiometric Bactec 460TB or Bactec 960 MGIT methods. In contrast, only one was susceptible by the proportion method on Löwenstein-Jensen medium and two on Middlebrook 7H10 agar. Low-level but probably clinically relevant RMP resistance linked to specific rpoB mutations is easily missed by standard growth-based methods, particularly the automated broth-based systems. Further studies are required to confirm these findings, to determine the frequency of these low-level-resistant isolates, and to identify technical improvements that may identify such strains. | - |
dc.description.statementofresponsibility | A. Van Deun, L. Barrera, I. Bastian, L. Fattorini, H. Hoffmann, K. M. Kam, L. Rigouts, S. Rüsch-Gerdes and A. Wright | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Microbiology | - |
dc.source.uri | http://dx.doi.org/10.1128/jcm.01209-09 | - |
dc.subject | Humans | - |
dc.subject | Mycobacterium tuberculosis | - |
dc.subject | Rifampin | - |
dc.subject | DNA-Directed RNA Polymerases | - |
dc.subject | Antitubercular Agents | - |
dc.subject | Microbial Sensitivity Tests | - |
dc.subject | Mutation, Missense | - |
dc.title | Mycobacterium tuberculosis strains with highly discordant rifampin susceptibility test results | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/JCM.01209-09 | - |
pubs.publication-status | Published | - |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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