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https://hdl.handle.net/2440/60647
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Type: | Journal article |
Title: | Effects of simulated bleeding in an in vitro nasal fibroblast wound healing model |
Author: | Beule, A. Athanasiadis, T. Field, J. Hosemann, W. Wormald, P. Tan, L. |
Citation: | American Journal of Rhinology and Allergy, 2010; 24(3):186-191 |
Publisher: | Ocean Side Publications Inc |
Issue Date: | 2010 |
ISSN: | 1945-8924 1945-8932 |
Statement of Responsibility: | Beule Achim Georg, Athanasiadis Theodore, Field John, Hosemann Werner, Wormald Peter-John and Tan Lor Wai |
Abstract: | Background: We investigated the effect of simulated bleeding on plasminogen activity, matrix metalloproteinase (MMP) expression, and wound healing using a human fibroblast model. Methods: Nasal fibroblasts from three chronic rhinosinusitis (CRS) patients with nasal polyps and three controls were grown in culture and a standardized injury was created using a punch. To mimic bleeding, fibroblasts were stimulated with plasminogen (100 μg/mL), plasminogen + tranexamic acid (TA; 100 μg/mL) or media only. At 24, 48, and 72 hours after injury, we measured urokinase plasminogen activator (uPA) and tissue-type plasminogen activator (tPA) activities and inactive and active MMP-2 and -9 expression. Results: Injury stimulated the nasal fibroblasts to express uPA and tPA and active and inactive MMP-2 and -9. In CRS patients, plasminogen significantly decreased MMP-9 expression after 48 hours (p < 0.04). In untreated fibroblasts, we observed a decrease in active MMP-9 expression, whereas plasminogen increased active MMP-9 expression after 48 hours (p < 0.04). At 24 hours, active MMP-9 expression was reduced by plasminogen ± TA (p < 0.02). Plasminogen also stimulated uPA expression in CRS patient fibroblasts after 48 hours (p < 0.04). Fibroblast proliferation occurred when exposed to plasminogen and was strongly modulated by uPA and inactive and active MMP-2. The quality of wound healing was affected by inactive MMP-2, uPA and tPA, simulation, and inhibition of bleeding. Conclusion: Activation of the plasminogen pathway and inactive MMP-2 expression tended to increase both proliferation of nasal fibroblasts and MMP-9 expression as a marker for deterioration of the quality of wound healing. |
Keywords: | Bleeding chronic rhinosinusitis endoscopic sinus surgery fibroblasts matrix metalloproteinases plasminogen tranexamic acid wound healing model |
Rights: | Website © 2010 Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article. |
DOI: | 10.2500/ajra.2010.24.3452 |
Published version: | http://dx.doi.org/10.2500/ajra.2010.24.3452 |
Appears in Collections: | Aurora harvest 5 Surgery publications |
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