Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/60688
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Type: | Journal article |
Title: | Modulation of CP2 family transcriptional activity by CRTR-1 and sumoylation |
Author: | To, S. Rodda, S. Rathjen, P. Keough, R. |
Citation: | PLoS One, 2010; 5(7):1-13 |
Publisher: | Public Library of Science |
Issue Date: | 2010 |
ISSN: | 1932-6203 1932-6203 |
Editor: | Whitsett, J.A. |
Statement of Responsibility: | Sarah To, Stephen J. Rodda, Peter D. Rathjen and Rebecca A. Keough |
Abstract: | CRTR-1 is a member of the CP2 family of transcription factors. Unlike other members of the family which are widely expressed, CRTR-1 expression shows specific spatio-temporal regulation. Gene targeting demonstrates that CRTR-1 plays a central role in the maturation and function of the salivary glands and the kidney. CRTR-1 has also recently been identified as a component of the complex transcriptional network that maintains pluripotency in embryonic stem (ES) cells. CRTR-1 was previously shown to be a repressor of transcription. We examine the activity of CRTR-1 in ES and other cells and show that CRTR-1 is generally an activator of transcription and that it modulates the activity of other family members, CP2, NF2d9 and altNF2d9, in a cell specific manner. We also demonstrate that CRTR-1 activity is regulated by sumoylation at a single major site, residue K30. These findings imply that functional redundancy with other family members may mask important roles for CRTR-1 in other tissues, including the blastocyst stage embryo and embryonic stem cells. |
Keywords: | Cell Line COS Cells Animals Humans DNA-Binding Proteins Transcription Factors Repressor Proteins Blotting, Western Electrophoretic Mobility Shift Assay Immunoprecipitation Protein Binding Chlorocebus aethiops |
Rights: | © 2010 To et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
DOI: | 10.1371/journal.pone.0011702 |
Published version: | http://dx.doi.org/10.1371/journal.pone.0011702 |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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