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|Title:||Evidence that vitamin D-3 promotes mast cell-dependent reduction of chronic UVB-nduced skin pathology in mice|
|Citation:||Journal of Experimental Medicine, 2010; 207(3):455-463|
|Publisher:||Rockefeller Univ Press|
|Lisa Biggs, Chunping Yu, Boris Fedoric, Angel F. Lopez, Stephen J. Galli and Michele A. Grimbaldeston|
|Abstract:||Mast cell production of interleukin-10 (IL-10) can limit the skin pathology induced by chronic low-dose ultraviolet (UV)-B irradiation. Although the mechanism that promotes mast cell IL-10 production in this setting is unknown, exposure of the skin to UVB irradiation induces increased production of the immune modifying agent 1alpha,25-dihydroxyvitamin D(3) (1alpha,25[OH](2)D(3)). We now show that 1alpha,25(OH)(2)D(3) can up-regulate IL-10 mRNA expression and induce IL-10 secretion in mouse mast cells in vitro. To investigate the roles of 1alpha,25(OH)(2)D(3) and mast cell vitamin D receptor (VDR) expression in chronically UVB-irradiated skin in vivo, we engrafted the skin of genetically mast cell-deficient WBB6F(1)-Kit(W/W-v) mice with bone marrow-derived cultured mast cells derived from C57BL/6 wild-type or VDR(-/-) mice. Optimal mast cell-dependent suppression of the inflammation, local production of proinflammatory cytokines, epidermal hyperplasia, and epidermal ulceration associated with chronic UVB irradiation of the skin in Kit(W/W-v) mice required expression of VDR by the adoptively transferred mast cells. Our findings suggest that 1alpha,25(OH)(2)D(3)/VDR-dependent induction of IL-10 production by cutaneous mast cells can contribute to the mast cell's ability to suppress inflammation and skin pathology at sites of chronic UVB irradiation.|
|Keywords:||Bone Marrow Cells|
Mice, Inbred C57BL
Cell Culture Techniques
|Rights:||© 2010 Biggs et al. Copyright © 2010 by The Rockefeller University Press.|
|Appears in Collections:||Aurora harvest|
Molecular and Biomedical Science publications
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