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https://hdl.handle.net/2440/63730
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dc.contributor.author | Pidot, S. | - |
dc.contributor.author | Porter, J. | - |
dc.contributor.author | Tobias, N. | - |
dc.contributor.author | Anderson, J. | - |
dc.contributor.author | Catmull, D. | - |
dc.contributor.author | Seemann, T. | - |
dc.contributor.author | Kidd, S. | - |
dc.contributor.author | Davies, J. | - |
dc.contributor.author | Reynolds, E. | - |
dc.contributor.author | Dashper, S. | - |
dc.contributor.author | Stinear, T. | - |
dc.date.issued | 2010 | - |
dc.identifier.citation | Molecular Microbiology, 2010; 78(5):1216-1231 | - |
dc.identifier.issn | 0950-382X | - |
dc.identifier.issn | 1365-2958 | - |
dc.identifier.uri | http://hdl.handle.net/2440/63730 | - |
dc.description.abstract | Mycobacterium ulcerans is the causative agent of the debilitating skin disease Buruli ulcer, which is most prevalent in Western and Central Africa. M. ulcerans shares >98% DNA sequence identity with Mycobacterium marinum, however, M. marinum produces granulomatous, but not ulcerative, lesions in humans and animals. Here we report the differential expression of a small heat shock protein (Hsp18) between strains of M. ulcerans (Hsp18(+) ) and M. marinum (Hsp18(-) ) and describe the molecular basis for this difference. We show by gene deletion and GFP reporter assays in M. marinum that a divergently transcribed gene called hspR_2, immediately upstream of hsp18, encodes a MerR-like regulatory protein that represses hsp18 transcription while promoting its own expression. Naturally occurring mutations within a 70 bp segment of the 144 bp hspR_2-hsp18 intergenic region among M. ulcerans strains inhibit hspR_2 transcription and explain the Hsp18(+) phenotype. We also propose a biological role for Hsp18, as we show that this protein significantly enhances bacterial attachment or aggregation during biofilm formation. This study has uncovered a new member of the MerR family of transcriptional regulators and suggests that upregulation of hsp18 expression was an important pathoadaptive response in the evolution of M. ulcerans from a M. marinum-like ancestor. | - |
dc.description.statementofresponsibility | Sacha J. Pidot, Jessica L. Porter, Nicholas J. Tobias, Jeffrey Anderson, Deanne Catmull, Torsten Seemann, Stephen Kidd, John K. Davies, Eric Reynolds, Stuart Dashper and Timothy P. Stinear | - |
dc.language.iso | en | - |
dc.publisher | Blackwell Publishing Ltd | - |
dc.rights | © 2010 Blackwell Publishing Ltd. | - |
dc.source.uri | http://dx.doi.org/10.1111/j.1365-2958.2010.07401.x | - |
dc.subject | Biofilms | - |
dc.subject | Mycobacterium marinum | - |
dc.subject | Mycobacterium ulcerans | - |
dc.subject | Bacterial Proteins | - |
dc.subject | alpha-Crystallins | - |
dc.subject | Heat-Shock Proteins | - |
dc.subject | Phylogeny | - |
dc.subject | Gene Expression Regulation, Bacterial | - |
dc.subject | Up-Regulation | - |
dc.subject | Base Sequence | - |
dc.subject | Molecular Sequence Data | - |
dc.subject | Promoter Regions, Genetic | - |
dc.title | Regulation of the 18 kDa heat shock protein in Mycobacterium ulcerans: An alpha-crystallin orthologue that promotes biofilm formation | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1111/j.1365-2958.2010.07401.x | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Kidd, S. [0000-0002-2118-1651] | - |
Appears in Collections: | Aurora harvest 5 Molecular and Biomedical Science publications |
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