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Type: Journal article
Title: Extracellular matrix formation enhances the ability of streptococcus pneumoniae to cause invasive disease
Author: Trappetti, C.
Ogunniyi, A.
Oggioni, M.
Paton, J.
Citation: PLoS One, 2011; 6(5):1-17
Publisher: Public Library of Science
Issue Date: 2011
ISSN: 1932-6203
Statement of
Claudia Trappetti, Abiodun D. Ogunniyi, Marco R. Oggioni and James C. Paton
Abstract: During infection, pneumococci exist mainly in sessile biofilms rather than in planktonic form, except during sepsis. However, relatively little is known about how biofilms contribute to pneumococcal pathogenesis. Here, we carried out a biofilm assay on opaque and transparent variants of a clinical serotype 19F strain WCH159. After 4 days incubation, scanning electron microscopy revealed that opaque biofilm bacteria produced an extracellular matrix, whereas the transparent variant did not. The opaque biofilm-derived bacteria translocated from the nasopharynx to the lungs and brain of mice, and showed 100- fold greater in vitro adherence to A549 cells than transparent bacteria. Microarray analysis of planktonic and sessile bacteria from transparent and opaque variants showed differential gene expression in two operons: the lic operon, which is involved in choline uptake, and in the two-component system, ciaRH. Mutants of these genes did not form an extracellular matrix, could not translocate from the nasopharynx to the lungs or the brain, and adhered poorly to A549 cells. We conclude that only the opaque phenotype is able to form extracellular matrix, and that the lic operon and ciaRH contribute to this process. We propose that during infection, extracellular matrix formation enhances the ability of pneumococci to cause invasive disease.
Keywords: Cell Line; Extracellular Matrix; Animals; Humans; Mice; Biofilms; Streptococcus pneumoniae; Pneumococcal Infections; Disease Models, Animal; Bacterial Capsules; Bacterial Proteins; Blotting, Western; Enzyme-Linked Immunosorbent Assay; Biological Assay; Colony Count, Microbial; Administration, Intranasal; Bacterial Adhesion; Gene Expression Regulation, Bacterial; Phenotype; Mutation
Description: Extent: 17p.
Rights: © 2011 Trappetti et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
RMID: 0020110849
DOI: 10.1371/journal.pone.0019844
Appears in Collections:Molecular and Biomedical Science publications

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