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Type: Journal article
Title: Rosiglitazone increases the expression of peroxisome proliferator-activated receptor-γ target genes in adipose tissue, liver, and skeletal muscle in the sheep fetus in late gestation
Other Titles: Rosiglitazone increases the expression of peroxisome proliferator-activated receptor-gamma target genes in adipose tissue, liver, and skeletal muscle in the sheep fetus in late gestation
Author: Muhlhausler, B.
Morrison, J.
McMillen, I.
Citation: Endocrinology, 2009; 150(9):4287-4294
Publisher: Endocrine Soc
Issue Date: 2009
ISSN: 0013-7227
Statement of
B. S. Muhlhausler, J. L. Morrison, and I. C. McMillen
Abstract: Exposure to maternal overnutrition increases the expression of peroxisome proliferator-activated receptor-γ (PPARγ) in adipose tissue before birth, and it has been proposed that the precocial activation of PPARγ target genes may lead to increased fat deposition in postnatal life. In this study, we determined the effect of intrafetal administration of a PPARγ agonist, rosiglitazone, on PPARγ target gene expression in fetal adipose tissue as well indirect actions of rosiglitazone on fetal liver and skeletal muscle. Osmotic pumps containing rosiglitazone (n = 7) or vehicle (15% ethanol, n = 7) were implanted into fetuses at 123–126 d gestation (term = 150 ± 3 d gestation). At 137–141 d gestation, tissues were collected and mRNA expression of PPARγ, lipoprotein lipase (LPL), adiponectin, and glycerol-3-phosphate dehydrogenase (G3PDH) in adipose tissue, PPARα and PPARγ-coactivator 1α (PGC1α) in liver and muscle and phosphoenolpyruvate carboxykinase (PEPCK) in liver determined by quantitative real-time RT-PCR. Plasma insulin concentrations were lower in rosiglitazone-treated fetuses (P < 0.02). Rosiglitazone treatment resulted in increased expression of LPL and adiponectin mRNA (P < 0.01) in fetal adipose tissue. The expression of PPARα mRNA in liver (P < 0.05) and PGC1α mRNA (P < 0.02) in skeletal muscle were also increased by rosiglitazone treatment. Rosiglitazone treatment increased expression of PPARγ target genes within fetal adipose tissue and also had direct or indirect actions on the fetal liver and muscle. The effects of activating PPARγ in fetal adipose tissue mimic those induced by prenatal overnutrition, and it is therefore possible that activation of PPARγ may be the initiating mechanism in the pathway from prenatal overnutrition to postnatal obesity.
Keywords: Muscle, Skeletal; Liver; Adipose Tissue; Fetus; Animals; Sheep; Thiazolidinediones; Insulin; Leptin; Lipoprotein Lipase; Phosphoenolpyruvate Carboxykinase (GTP); Trans-Activators; PPAR alpha; PPAR gamma; RNA, Messenger; Pregnancy; Pregnancy, Animal; Female; Glycerol-3-Phosphate Dehydrogenase (NAD+); Adiponectin; Maternal Nutritional Physiological Phenomena
Rights: Copyright © 2009 by The Endocrine Society
RMID: 0020108836
DOI: 10.1210/en.2009-0462
Appears in Collections:Molecular and Biomedical Science publications

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