Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/6775
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Type: Journal article
Title: The osteoclastogenic molecules RANKL and RANK are associated with periprosthetic osteolysis
Author: Haynes, D.
Crotti, T.
Potter, A.
Loric, M.
Atkins, G.
Howie, D.
Findlay, D.
Citation: Journal of Bone and Joint Surgery-British Volume, 2001; 83B(6):902-911
Publisher: British Editorial Soc Bone Joint Surgery
Issue Date: 2001
ISSN: 0301-620X
2044-5377
Statement of
Responsibility: 
D. R. Haynes, T. N. Crotti, A. E. Potter, M. Loric, G. J. Atkins, D. W. Howie, and D. M. Findlay
Abstract: Extensive osteolysis adjacent to implants is often associated with wear particles of prosthetic material. We have investigated if RANKL, also known as osteoprotegerin ligand, osteoclast differentiation factor or TRANCE, and its natural inhibitor, osteoprotegerin (OPG), may be important in controlling this bone loss. Cells isolated from periprosthetic tissues containing wear particles expressed mRNA encoding for the pro-osteoclastogenic molecules, RANKL, its receptor RANK, monocyte colony-stimulating factor (M-CSF), interleukin (IL)-1ß, tumour necrosis factor (TNF), IL-6, and soluble IL-6 receptor, as well as OPG. Osteoclasts formed from cells isolated from periprosthetic tissues in the presence and absence of human osteoblastic cells. When osteoclasts formed in the absence of osteoblastic cells, markedly higher levels of RANKL mRNA relative to OPG mRNA were expressed. Particles of prosthetic materials also stimulated human monocytes to express osteoclastogenic molecules in vitro. Our results suggest that ingestion of prosthetic wear particles by macrophages results in expression of osteoclast-differentiating molecules and the stimulation of macrophage differentiation into osteoclasts.
Keywords: Cells, Cultured; Macrophages; Osteoclasts; Osteolysis; Glycoproteins; Tumor Necrosis Factor-alpha; Carrier Proteins; Macrophage Colony-Stimulating Factor; Membrane Glycoproteins; Receptors, Tumor Necrosis Factor; Receptors, Cytoplasmic and Nuclear; RNA, Messenger; Interleukin-6; Cytokines; Reverse Transcriptase Polymerase Chain Reaction; RANK Ligand; Receptor Activator of Nuclear Factor-kappa B; Osteoprotegerin
Rights: Copyright © 2001 by British Editorial Society of Bone and Joint Surgery
RMID: 0020010976
DOI: 10.1302/0301-620X.83B6.10905
Published version: http://www.jbjs.org.uk/cgi/content/abstract/83-B/6/902
Appears in Collections:Orthopaedics and Trauma publications
Pathology publications

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