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https://hdl.handle.net/2440/6783
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dc.contributor.author | Gronthos, S. | - |
dc.contributor.author | Zannettino, A. | - |
dc.contributor.author | Graves, S. | - |
dc.contributor.author | Ohta, S. | - |
dc.contributor.author | Hay, S. | - |
dc.contributor.author | Simmons, P. | - |
dc.date.issued | 1999 | - |
dc.identifier.citation | Journal of Bone and Mineral Research, 1999; 14(1):47-56 | - |
dc.identifier.issn | 0884-0431 | - |
dc.identifier.issn | 1523-4681 | - |
dc.identifier.uri | http://hdl.handle.net/2440/6783 | - |
dc.description.abstract | Human osteoblast-like cells can be readily cultured from explants of trabecular bone, reproducibly expressing the characteristics of cells belonging to the osteoblastic lineage. Dual-color fluorescence-activated cell sorting was employed to develop a model of bone cell development in primary cultures of normal human bone cells (NHBCs) based on the cell surface expression of the stromal precursor cell marker STRO-1 and the osteoblastic marker alkaline phosphatase (ALP). Cells expressing the STRO-1 antigen exclusively (STRO-1+/ALP-), were found to exhibit qualities preosteoblastic in nature both functionally by their reduced ability to form a mineralized bone matrix over time, as measured by calcium release assay, and in the lack of their expression of various bone-related markers including bone sialoprotein, osteopontin, and parathyroid hormone receptor based on reverse trancriptase polymerase chain reaction (PCR) analysis. The majority of the NHBCs which expressed the STRO-1-/ALP+ and STRO-1-/ALP- phenotypes appeared to represent fully differentiated osteoblasts, while the STRO-1+/ALP+ subset represented an intermediate preosteoblastic stage of development. All STRO-1/ALP NHBC subsets were also found to express the DNA-binding transcription factor CBFA-1, confirming that these cultures represent committed osteogenic cells. In addition, our primer sets yielded four distinct alternative splice variants of the expected PCR product for CBFA-1 in each of the STRO-1/ALP subsets, with the exception of the proposed preosteoblastic STRO-1+/ALP- subpopulation. Furthermore, upon re-culture of the four different STRO-1/ALP subsets only the STRO-1+/ALP- subpopulation was able to give rise to all of the four subsets yielding the same proportions of STRO-1/ALP expression as in the original primary cultures. The data presented in this study demonstrate a hierarchy of bone cell development in vitro and facilitate the study of bone cell differentiation and function. | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.source.uri | http://dx.doi.org/10.1359/jbmr.1999.14.1.47 | - |
dc.subject | Bone Matrix | - |
dc.subject | Cells, Cultured | - |
dc.subject | Humans | - |
dc.subject | Alkaline Phosphatase | - |
dc.subject | Transcription Factors | - |
dc.subject | Antibodies, Monoclonal | - |
dc.subject | Antigens, Surface | - |
dc.subject | Cell Lineage | - |
dc.subject | Bone Development | - |
dc.subject | Osteogenesis | - |
dc.subject | Bone Density | - |
dc.subject | Phenotype | - |
dc.subject | Reference Values | - |
dc.subject | Aged | - |
dc.subject | Aged, 80 and over | - |
dc.subject | Middle Aged | - |
dc.subject | Biomarkers | - |
dc.title | Differential cell surface expression of the STRO-1 and alkaline phosphatase antigens on discrete developmental stages in primary cultures of human bone cells | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1359/jbmr.1999.14.1.47 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Gronthos, S. [0000-0002-6225-3084] | - |
dc.identifier.orcid | Zannettino, A. [0000-0002-6646-6167] | - |
dc.identifier.orcid | Graves, S. [0000-0002-1629-319X] | - |
Appears in Collections: | Aurora harvest Orthopaedics and Trauma publications |
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