Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/6813
Citations | ||
Scopus | Web of Science® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Intracellular zinc depletion induces caspase activation and p21Waf1/Cip1 cleavage in human epithelial cell lines |
Author: | Chai, F. TruongTran, A. Evdokiou, A. Young, G. Zalewski, P. |
Citation: | Journal of Infectious Diseases, 2000; 182(Suppl 1):S85-S92 |
Publisher: | University of Chicago Press |
Issue Date: | 2000 |
ISSN: | 0022-1899 1537-6613 |
Statement of Responsibility: | F. Chai, A. Q. Truong-Tran, A. Evdokiou, G. P. Young and P. D. Zalewski |
Abstract: | To better understand the mechanisms by which zinc deficiency induces epithelial cell death, studies were done of the effects of intracellular zinc depletion induced by the zinc chelator TPEN on apoptosis-related events in human malignant epithelial cell lines LIM1215 (colonic), NCI-H292 (bronchial), and A549 (alveolar type II). In TPEN-treated cells, depletion of zinc was followed by activation of caspase-3 (as demonstrated by enzymatic assay and Western blotting), DNA fragmentation, and morphologic changes. Increase in caspase-3 activity began 1–2 h after addition of TPEN, suggesting that zinc may suppress a step just before the activation of this caspase. Caspase-6, a mediator of caspase-3 processing, also increased, but later than caspase-3. Effects of TPEN on apoptosis were completely prevented by exogenous ZnSO4 and partially prevented by peptide caspase inhibitors. A critical substrate of caspase-3 may be the cell cycle regulator p21Waf1/Cip1, which was rapidly cleaved in TPEN-treated cells to a 15-kDa fragment before further degradation. |
Keywords: | Tumor Cells, Cultured Epithelial Cells Humans Zinc Ethylenediamines Caspases Cyclins Chelating Agents Enzyme Inhibitors Cysteine Proteinase Inhibitors Apoptosis DNA Fragmentation Kinetics Cyclin-Dependent Kinase Inhibitor p21 Caspase 3 Caspase 6 |
Rights: | © 2000 by the Infectious Diseases Society of America. All rights reserved. |
DOI: | 10.1086/315914 |
Published version: | http://dx.doi.org/10.1086/315914 |
Appears in Collections: | Aurora harvest 5 Orthopaedics and Trauma publications |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
hdl_6813.pdf | 803.49 kB | Publisher's PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.