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https://hdl.handle.net/2440/68226
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Type: | Journal article |
Title: | The molecular basis of filamin binding to integrins and competition with talin |
Author: | Kiema, T. Lad, Y. Jiang, P. Oxley, C. Baldassarre, M. Wegener, K. Campbell, I. Ylanne, J. Calderwood, D. |
Citation: | Molecular Cell, 2006; 21(3):337-347 |
Publisher: | Cell Press |
Issue Date: | 2006 |
ISSN: | 1097-2765 1097-4164 |
Statement of Responsibility: | Tiila Kiema, Yatish Lad, Pengju Jiang, Camilla L. Oxley, Massimiliano Baldassarre, Kate L. Wegener, Iain D. Campbell, Jari Ylänne and David A. Calderwood |
Abstract: | The ability of adhesion receptors to transmit biochemical signals and mechanical force across cell membranes depends on interactions with the actin cytoskeleton. Filamins are large, actin-crosslinking proteins that connect multiple transmembrane and signaling proteins to the cytoskeleton. Here, we describe the high-resolution structure of an interface between filamin A and an integrin adhesion receptor. When bound, the integrin β cytoplasmic tail forms an extended β strand that interacts with β strands C and D of the filamin immunoglobulin-like domain (IgFLN) 21. This interface is common to many integrins, and we suggest it is a prototype for other IgFLN domain interactions. Notably, the structurally defined filamin binding site overlaps with that of the integrin-regulator talin, and these proteins compete for binding to integrin tails, allowing integrin-filamin interactions to impact talin-dependent integrin activation. Phosphothreonine-mimicking mutations inhibit filamin, but not talin, binding, indicating that kinases may modulate this competition and provide additional means to control integrin functions. |
Keywords: | NIH 3T3 Cells Animals Mice Microfilament Proteins Calpain Contractile Proteins Talin Integrin beta Chains Recombinant Fusion Proteins Crystallography, X-Ray Nuclear Magnetic Resonance, Biomolecular Reproducibility of Results Binding Sites Amino Acid Sequence Protein Conformation Protein Structure, Tertiary Protein Binding Sequence Homology, Amino Acid Models, Molecular Molecular Sequence Data Filamins |
Rights: | Copyright 2006 Elsevier Inc. |
DOI: | 10.1016/j.molcel.2006.01.011 |
Description (link): | http://www.sciencedirect.com/science/journal/10972765 |
Published version: | http://dx.doi.org/10.1016/j.molcel.2006.01.011 |
Appears in Collections: | Aurora harvest Molecular and Biomedical Science publications |
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