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Type: Journal article
Title: Conservation of plastid sequences in the plant nuclear genome for millions of years facilitates endosymbiotic evolution
Author: Rousseau, M.
Ayliffe, M.
Timmis, J.
Citation: Plant Physiology, 2011; 157(4):2181-2193
Publisher: Amer Soc Plant Physiologists
Issue Date: 2011
ISSN: 0032-0889
Statement of
Mathieu Rousseau-Gueutin, Michael A. Ayliffe, and Jeremy N. Timmis
Abstract: The nuclear genome of eukaryotes contains large amounts of cytoplasmic organelle DNA (nuclear integrants of organelle DNA [norgs]). The recent sequencing of many mitochondrial and chloroplast genomes has enabled investigation of the potential role of norgs in endosymbiotic evolution. In this article, we describe a new polymerase chain reaction-based method that allows the identification and evolutionary study of recent and older norgs in a range of eukaryotes. We tested this method in the genus Nicotiana and obtained sequences from seven nuclear integrants of plastid DNA (nupts) totaling 25 kb in length. These nupts were estimated to have been transferred 0.033 to 5.81 million years ago. The spectrum of mutations present in the potential protein-coding sequences compared with the noncoding sequences of each nupt revealed that nupts evolve in a nuclear-specific manner and are under neutral evolution. Indels were more frequent in noncoding regions than in potential coding sequences of former chloroplastic DNA, most probably due to the presence of a higher number of homopolymeric sequences. Unexpectedly, some potential protein-coding sequences within the nupts still contained intact open reading frames for up to 5.81 million years. These results suggest that chloroplast genes transferred to the nucleus have in some cases several millions of years to acquire nuclear regulatory elements and become functional. The different factors influencing this time frame and the potential role of nupts in endosymbiotic gene transfer are discussed.
Keywords: Cell Nucleus
DNA, Chloroplast
DNA, Plant
DNA Primers
Polymerase Chain Reaction
Sequence Alignment
Sequence Analysis, DNA
Evolution, Molecular
Base Sequence
Genes, Plant
Genome, Plant
Time Factors
Molecular Sequence Data
Genome, Chloroplast
Rights: Copyright 2011 American Society of Plant Biologists. All Rights Reserved.
DOI: 10.1104/pp.111.185074
Grant ID:
Appears in Collections:Aurora harvest
Molecular and Biomedical Science publications

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