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Type: Journal article
Title: Genetic mapping of Bt-toxin binding proteins in a Cry1A-toxin resistant strain of diamondback moth Plutella xylostella
Author: Baxter, S.
Zhao, J.
Shelton, A.
Vogel, H.
Heckel, D.
Citation: Insect Biochemistry and Molecular Biology, 2008; 38(2):125-135
Publisher: Pergamon-Elsevier Science Ltd
Issue Date: 2008
ISSN: 0965-1748
Statement of
Simon W. Baxter, Jian-Zhou Zhao, Anthony M. Shelton, Heiko Vogel, David G. Heckel
Abstract: A major mechanism of resistance to Bacillus thuringiensis (Bt) toxins in Lepidoptera is a reduction of toxin binding to sites in the midgut membrane. Genetic studies of three different species have shown that mutations in a candidate Bt receptor, a 12-cadherin-domain protein, confer Cry1A toxin resistance. Despite a similar resistance profile in a fourth lepidopteran species, Plutella xylostella, we have previously shown that the cadherin orthologue maps to a different linkage group (LG8) than Cry1Ac resistance (LG22). Here we tested the hypothesis that mutations in other genes encoding candidate Bt-binding targets could be responsible for Bt resistance, by mapping eight aminopeptidases, an alkaline phosphatase (ALP), an intestinal mucin, and a P252 glycoprotein with respect to the 29 AFLP marked linkage groups in a P. xylostella cross segregating for Cry1Ac resistance. A homologue of the Caenorhabditis elegans Bt resistance gene bre-2 was also mapped. None of the genes analysed were on the same chromosome containing the Cry1Ac resistance locus, eliminating them as candidate resistance genes in the parental resistant strain SC1. Although this finding excludes cis-acting mutations in these genes as causing resistance in this strain, one or more of the expressed proteins may still bind Cry1Ac toxin, and post-translational modifications could affect this binding and thereby exert a trans-acting effect on resistance.
Keywords: Bacillus thuringiensis (Bt)
Plutella xylostella
Diamondback moth
Linkage mapping
Rights: Copyright © 2007 Elsevier Ltd. All rights reserved.
DOI: 10.1016/j.ibmb.2007.09.014
Appears in Collections:Aurora harvest
Environment Institute publications
Molecular and Biomedical Science publications

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