Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Genetic mapping of Bt-toxin binding proteins in a Cry1A-toxin resistant strain of diamondback moth Plutella xylostella|
|Citation:||Insect Biochemistry and Molecular Biology, 2008; 38(2):125-135|
|Publisher:||Pergamon-Elsevier Science Ltd|
|Simon W. Baxter, Jian-Zhou Zhao, Anthony M. Shelton, Heiko Vogel, David G. Heckel|
|Abstract:||A major mechanism of resistance to Bacillus thuringiensis (Bt) toxins in Lepidoptera is a reduction of toxin binding to sites in the midgut membrane. Genetic studies of three different species have shown that mutations in a candidate Bt receptor, a 12-cadherin-domain protein, confer Cry1A toxin resistance. Despite a similar resistance profile in a fourth lepidopteran species, Plutella xylostella, we have previously shown that the cadherin orthologue maps to a different linkage group (LG8) than Cry1Ac resistance (LG22). Here we tested the hypothesis that mutations in other genes encoding candidate Bt-binding targets could be responsible for Bt resistance, by mapping eight aminopeptidases, an alkaline phosphatase (ALP), an intestinal mucin, and a P252 glycoprotein with respect to the 29 AFLP marked linkage groups in a P. xylostella cross segregating for Cry1Ac resistance. A homologue of the Caenorhabditis elegans Bt resistance gene bre-2 was also mapped. None of the genes analysed were on the same chromosome containing the Cry1Ac resistance locus, eliminating them as candidate resistance genes in the parental resistant strain SC1. Although this finding excludes cis-acting mutations in these genes as causing resistance in this strain, one or more of the expressed proteins may still bind Cry1Ac toxin, and post-translational modifications could affect this binding and thereby exert a trans-acting effect on resistance.|
|Keywords:||Bacillus thuringiensis (Bt); Plutella xylostella; Diamondback moth; Insecticide; Resistance; Linkage mapping|
|Rights:||Copyright © 2007 Elsevier Ltd. All rights reserved.|
|Appears in Collections:||Molecular and Biomedical Science publications|
Environment Institute publications
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.