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|Title:||Matrix-assisted laser desorption/ionization imaging protocol for in situ characterization of tryptic peptide identity and distribution in formalin-fixed tissue|
|Citation:||Rapid Communications in Mass Spectrometry, 2013; 27(6):655-670|
|Publisher:||John Wiley & Sons Ltd|
|Ove J. R. Gustafsson, James S. Eddes, Stephan Meding, Shaun R. McColl, Martin K. Oehler and Peter Hoffmann|
|Abstract:||<h4>Rationale</h4>Matrix-assisted laser desorption/ionization (MALDI) imaging mass spectrometry provides the means to map the in situ distribution of tryptic peptides in formalin-fixed clinical tissue samples. The ability to analyze clinical samples is of great importance to further developments in the imaging field. However, there is a requirement in this field of research for additional methods describing the characterization of tryptic peptides by MALDI imaging.<h4>Methods and results</h4>This protocol gives highly detailed instructions, with examples, for (1) successfully performing tryptic peptide MALDI imaging on formalin-fixed cancer tissue using a MALDI-TOF/TOF MS instrument, (2) tentatively generating identifications through nLC/MS/MS, and (3) validating these identifications by in situ MS/MS of peptides of interest.<h4>Conclusions</h4>This protocol provides a detailed and straightforward description of the methods required for groups new to MALDI imaging to begin analysis of formalin-fixed clinical samples.|
|Keywords:||Humans; Neoplasms; Formaldehyde; Trypsin; Peptide Fragments; Calibration; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Histocytological Preparation Techniques; Amino Acid Sequence; Image Processing, Computer-Assisted; Molecular Sequence Data; Tandem Mass Spectrometry; Molecular Imaging|
|Rights:||Copyright © 2013 John Wiley & Sons, Ltd.|
|Appears in Collections:||IPAS publications|
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