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https://hdl.handle.net/2440/78984
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Type: | Journal article |
Title: | Expression of peptidylarginine deiminase-2 and -4, citrullinated proteins and anti-citrullinated protein antibodies in human gingiva |
Author: | Harvey, G. Fitzsimmons, T. Dharmapatni, A. Marchant, C. Haynes, D. Bartold, P. |
Citation: | Journal of Periodontal Research, 2013; 48(2):252-261 |
Publisher: | Blackwell Munksgaard |
Issue Date: | 2013 |
ISSN: | 0022-3484 1600-0765 |
Statement of Responsibility: | G. P. Harvey, T. R. Fitzsimmons, A. A. S. S. K. Dhamarpatni, C. Marchant, D. R. Haynes, P. M. Bartold |
Abstract: | BACKGROUND AND OBJECTIVE The presence of citrullinated proteins, and peptidylarginine deiminase types -2 (PAD-2) and -4 (PAD-4) in periodontal tissues, determine the presence of anti-cyclic citrullinated protein antibodies (anti-CCP) in gingival crevicular fluid (GCF) and compare the expression of these proteins between inflamed and non-inflamed sites. MATERIAL AND METHODS Tissue sections were stained using antibodies against citrullinated proteins, PAD-2 and PAD-4. RT-PCR was performed to investigate PAD-2 and PAD-4 mRNA in inflamed and non-inflamed gingival tissues. Anti-CCP antibodies in gingival crevicular fluid were detected by ELISA. RESULTS Citrullinated proteins, PAD-2 and PAD-4 were detected in gingiva. There was a correlation between inflammation and expression of these proteins. mRNAs for PAD-2 and PAD-4 were detected in both inflamed and non-inflamed gingival tissues. Antibodies to CCP were found mostly in the GCF of individuals with periodontitis. Conclusion PAD-2 and PAD-4 (protein and mRNA) as well as citrullinated proteins are present in inflamed gingiva, and anti-CCP antibodies can be detected in the GCF of some patients. Tissue expression of citrullinated proteins and PAD increased with the severity of inflammation. The presence of anti-CCP antibodies in GCF was almost exclusive to a subset of patients with periodontitis. Increased expression of these proteins in inflamed gingiva lends support to the notion that periodontal inflammation contributes to the inflammatory burden in a similar way to rheumatoid arthritis. |
Keywords: | anti-citrullinated proteins chronic inflammation citrullination periodontitis rheumatoid arthritis |
Rights: | © 2012 John Wiley & Sons A/S |
DOI: | 10.1111/jre.12002 |
Published version: | http://dx.doi.org/10.1111/jre.12002 |
Appears in Collections: | Aurora harvest 4 Dentistry publications |
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