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Type: Journal article
Title: Expression of peptidylarginine deiminase-2 and -4, citrullinated proteins and anti-citrullinated protein antibodies in human gingiva
Author: Harvey, G.
Fitzsimmons, T.
Dharmapatni, A.
Marchant, C.
Haynes, D.
Bartold, P.
Citation: Journal of Periodontal Research, 2013; 48(2):252-261
Publisher: Blackwell Munksgaard
Issue Date: 2013
ISSN: 0022-3484
Statement of
G. P. Harvey, T. R. Fitzsimmons, A. A. S. S. K. Dhamarpatni, C. Marchant, D. R. Haynes, P. M. Bartold
Abstract: BACKGROUND AND OBJECTIVE The presence of citrullinated proteins, and peptidylarginine deiminase types -2 (PAD-2) and -4 (PAD-4) in periodontal tissues, determine the presence of anti-cyclic citrullinated protein antibodies (anti-CCP) in gingival crevicular fluid (GCF) and compare the expression of these proteins between inflamed and non-inflamed sites. MATERIAL AND METHODS Tissue sections were stained using antibodies against citrullinated proteins, PAD-2 and PAD-4. RT-PCR was performed to investigate PAD-2 and PAD-4 mRNA in inflamed and non-inflamed gingival tissues. Anti-CCP antibodies in gingival crevicular fluid were detected by ELISA. RESULTS Citrullinated proteins, PAD-2 and PAD-4 were detected in gingiva. There was a correlation between inflammation and expression of these proteins. mRNAs for PAD-2 and PAD-4 were detected in both inflamed and non-inflamed gingival tissues. Antibodies to CCP were found mostly in the GCF of individuals with periodontitis. Conclusion PAD-2 and PAD-4 (protein and mRNA) as well as citrullinated proteins are present in inflamed gingiva, and anti-CCP antibodies can be detected in the GCF of some patients. Tissue expression of citrullinated proteins and PAD increased with the severity of inflammation. The presence of anti-CCP antibodies in GCF was almost exclusive to a subset of patients with periodontitis. Increased expression of these proteins in inflamed gingiva lends support to the notion that periodontal inflammation contributes to the inflammatory burden in a similar way to rheumatoid arthritis.
Keywords: anti-citrullinated proteins; chronic inflammation; citrullination; periodontitis; rheumatoid arthritis
Rights: © 2012 John Wiley & Sons A/S
RMID: 0020125788
DOI: 10.1111/jre.12002
Appears in Collections:Dentistry publications

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