Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/80896
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Type: Journal article
Title: The accuracy of Influenza A (H1N1) “swine flu” laboratory testing: a systematic review of diagnostic test accuracy
Author: White, S.
Schultz, T.
Citation: JBI Database of Systematic Reviews and Implementation Reports, 2013; 11(4):67-114
Publisher: Joanna Briggs Institute
Issue Date: 2013
ISSN: 2202-4433
2202-4433
Department: Faculty of Health Sciences
Statement of
Responsibility: 
Sarahlouise White, Tim Schultz
Abstract: BACKGROUND Influenza A (H1N1) recently became pandemic, highlighting the need for a cheap and accurate diagnostic test to diagnose this virus in a clinically relevant timeframe. The current reference standard (viral culture) requires a significant degree of technical expertise, laboratory time, resources and can take up to ten days to obtain a result, during which time there could be a significant spread of infection. OBJECTIVES To obtain summary estimates of the diagnostic accuracy of currently available laboratory tests compared to viral culture for the diagnosis of Influenza A (H1N1) from respiratory samples. INCLUSION CRITERIA TYPES OF PARTICIPANTS Patients presenting with influenza-like illnesses and who underwent both an index and reference test specific for influenza A (H1N1). FOCUS OF THE REVIEW The diagnostic test accuracy of tests for laboratory tests for Influenza A (H1N1) TYPES OF STUDIES Diagnostic test accuracy studies that compared the diagnostic accuracy of any laboratory test (index test) compared to viral culture as the reference test were considered for inclusion. SEARCH STRATEGY Diagnostic tests are still poorly indexed by major databases; therefore the search strategy was deliberately very broad and was conducted across 14 major databases during May 2010. METHODOLOGICAL QUALITY Methodological quality was determined using the QUADAS checklist. Overall the methodological quality of the studies was moderate, however all suffered from incomplete reporting. DATA COLLECTION Data was collected to determine the accuracy of the index tests and completeness of the conduct and reporting. Patients were identified as being either H1N1 positive or negative on the basis of the viral culture results. The index test results were then compared for sensitivity and specificity. DATA SYNTHESIS Meta analysis was not appropriate as the included studies were heterogeneous, therefore data was synthesized in narrative summary. RESULTS The search identified 3843 studies. Of these 56 full text articles were retrieved for further analysis. Twenty nine articles were critically appraised, with 24 being excluded on the basis of incongruence with the review objective or for containing insufficient detail. The remaining five cross sectional studies examined the diagnostic accuracy of polymerase chain reaction (PCR), and were included. Two studies reported accuracy measures and when reported, the sensitivity and specificity of PCR was high. CONCLUSIONS Although unable to generate a summary estimate of the overall accuracy of PCR, the available evidence suggests that PCR using respiratory specimens appears to be highly sensitive and specific at identifying patients with H1N1.
Keywords: Influenza A; H1N1; swine flu; sensitivity; specificity; diagnostic test accuracy; viral culture; polymerase chain reaction; PCR
Rights: Copyright status unknown
RMID: 0020138109
DOI: 10.11124/01938924-201311040-00002
Published version: http://www.joannabriggslibrary.org/jbilibrary/index.php/jbisrir/article/view/964
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