Please use this identifier to cite or link to this item:
|Scopus||Web of Science®||Altmetric|
|Title:||Host components in hepatitis B antigen|
|Citation:||Journal of General Virology, 1975; 27(2):117-126|
|Publisher:||Society for General Microbiology|
|C. J. Burrell|
|Abstract:||Purified [125I]-labelled 20 to 25 nm hepatitis B antigen particles were found to give low affinity immunoprecipitation reactions with antisera to several normal serum components, which were immunologically distinct from the reaction due to the classical hepatitis B antigen surface determinant. These additional antigenic determinants were acid-stable and tightly bound to the particles; they could not be released by treatment with Tween 80 or ether, but were removed by protease digestion with the preservation of particle integrity. It was not possible to distinguish whether they were due to the presence of trace amounts of partly denatured serum components, or to a weak cross-reaction with antigens present in normal serum. The implications of this finding for hepatitis B antigen and antibody detection in sensititive assays are discussed. No evidence was found for native antigenic material, present in normal serum or normal liver cells, being integral to the structure of these particles.|
|Keywords:||Humans; Iodine Radioisotopes; Polysorbates; Bromelains; Trypsin; Lipoproteins; Serum Albumin; Immunoglobulin G; Immune Sera; Hepatitis B Antigens; Epitopes; Radioimmunoassay; Immunodiffusion; Cross Reactions|
|Rights:||Copyright status unknown|
|Appears in Collections:||Molecular and Biomedical Science publications|
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.