Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/86772
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Type: Journal article
Title: Porphyromonas gingivalis peptidylarginine deiminase, a key contributor in the pathogenesis of experimental periodontal disease and experimental arthritis
Author: Gully, N.
Bright, R.
Marino, V.
Marchant, C.
Cantley, M.
Haynes, D.
Butler, C.
Dashper, S.
Reynolds, E.
Bartold, M.
Citation: PLoS One, 2014; 9(6):e100838-1-e100838-12
Publisher: Public Library of Science
Issue Date: 2014
ISSN: 1932-6203
1932-6203
Editor: Yilmaz, Ö.
Statement of
Responsibility: 
Neville Gully, Richard Bright, Victor Marino, Ceilidh Marchant, Melissa Cantley, David Haynes, Catherine Butler, Stuart Dashper, Eric Reynolds, Mark Bartold
Abstract: OBJECTIVES: To investigate the suggested role of Porphyromonas gingivalis peptidylarginine deiminase (PAD) in the relationship between the aetiology of periodontal disease and experimentally induced arthritis and the possible association between these two conditions. METHODS: A genetically modified PAD-deficient strain of P. gingivalis W50 was produced. The effect of this strain, compared to the wild type, in an established murine model for experimental periodontitis and experimental arthritis was assessed. Experimental periodontitis was induced following oral inoculation with the PAD-deficient and wild type strains of P. gingivalis. Experimental arthritis was induced via the collagen antibody induction process and was monitored by assessment of paw swelling and micro-CT analysis of the radio-carpal joints. Experimental periodontitis was monitored by micro CT scans of the mandible and histological assessment of the periodontal tissues around the mandibular molars. Serum levels of anti-citrullinated protein antibodies (ACPA) and P. gingivalis were assessed by ELISA. RESULTS: The development of experimental periodontitis was significantly reduced in the presence of the PAD-deficient P. gingivalis strain. When experimental arthritis was induced in the presence of the PAD-deficient strain there was less paw swelling, less erosive bone damage to the joints and reduced serum ACPA levels when compared to the wild type P. gingivalis inoculated group. CONCLUSION: This study has demonstrated that a PAD-deficient strain of P. gingivalis was associated with significantly reduced periodontal inflammation. In addition the extent of experimental arthritis was significantly reduced in animals exposed to prior induction of periodontal disease through oral inoculation of the PAD-deficient strain versus the wild type. This adds further evidence to the potential role for P. gingivalis and its PAD in the pathogenesis of periodontitis and exacerbation of arthritis. Further studies are now needed to elucidate the mechanisms which drive these processes.
Keywords: Animals
Mice, Inbred BALB C
Mice
Porphyromonas gingivalis
Arthritis, Experimental
Periodontitis
Disease Models, Animal
Hydrolases
Bacterial Proteins
Radiography
Enzyme-Linked Immunosorbent Assay
Protein-Arginine Deiminases
Rights: © 2014 Gully et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0100838
Appears in Collections:Aurora harvest 2
Dentistry publications

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