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Type: Journal article
Title: Distribution and speciation of bromine in mammalian tissue and fluids by X-ray fluorescence imaging and X-ray absorption spectroscopy
Author: Ceko, M.
Hummitzsch, K.
Hatzirodos, N.
Bonner, W.
James, S.
Kirby, J.
Rodgers, R.
Harris, H.
Citation: Metallomics: integrated biometal science, 2015; 7(5):756-765
Publisher: Royal Society of Chemistry
Issue Date: 2015
ISSN: 1756-5901
Statement of
Melanie J. Ceko, Katja Hummitzsch, Nicholas Hatzirodos, Wendy Bonner, Simon A. James, Jason K. Kirby, Raymond J. Rodgers and Hugh H. Harris
Abstract: Bromine is one of the most abundant and ubiquitous trace elements in the biosphere and until recently had not been shown to perform any essential biological function in animals. A recent study demonstrated that bromine is required as a cofactor for peroxidasin-catalysed formation of sulfilimine crosslinks in Drosophila. In addition, bromine dietary deficiency is lethal in Drosophila, whereas bromine replenishment restores viability. The aim of this study was to examine the distribution and speciation of bromine in mammalian tissues and fluids to provide further insights into the role and function of this element in biological systems. In this study we used X-ray fluorescence (XRF) imaging and inductively coupled plasma-mass spectrometry (ICP-MS) to examine the distribution of bromine in bovine ovarian tissue samples, follicular fluid and aortic serum, as well as human whole blood and serum and X-ray absorption spectroscopy (XAS) to identify the chemical species of bromine in a range of mammalian tissue (bovine, ovine, porcine and murine), whole blood and serum samples (bovine, ovine, porcine, murine and human), and marine samples (salmon (Salmo salar), kingfish (Seriola lalandi) and Scleractinian coral). Bromine was found to be widely distributed across all tissues and fluids examined. In the bovine ovary in particular it was more concentrated in the sub-endothelial regions of arterioles. Statistical comparison of the near-edge region of the X-ray absorption spectra with a library of bromine standards led to the conclusion that the major form of bromine in all samples analysed was bromide.
Keywords: Ovary
Trace Elements
Tandem Mass Spectrometry
X-Ray Absorption Spectroscopy
Optical Imaging
Rights: This journal is © The Royal Society of Chemistry 2015
DOI: 10.1039/c4mt00338a
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