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Type: Journal article
Title: Transcription of nuclear organellar DNA in a model plant system
Author: Wang, D.
Qu, Z.
Adelson, D.
Zhu, J.
Timmis, J.
Citation: Genome Biology and Evolution, 2014; 6(6):1327-1334
Publisher: Oxford University Press
Issue Date: 2014
ISSN: 1759-6653
Statement of
Dong Wang, Zhipeng Qu, David L. Adelson, Jian-Kang Zhu, and Jeremy N. Timmis
Abstract: Endosymbiotic gene transfer from cytoplasmic organelles (chloroplasts and mitochondria) to the nucleus is an ongoing process in land plants. Although the frequency of organelle DNA migration is high, functional gene transfer is rare because a nuclear promoter is thought necessary for activity in the nucleus. Here we show that a chloroplast promoter, 16S rrn, drives nuclear transcription, suggesting that a transferred organellar gene may become active without obtaining a nuclear promoter. Examining the chromatin status of a known de novo chloroplast integrant indicates that plastid DNA inserts into open chromatin and that this relaxed condition is maintained after integration. Transcription of nuclear organelle DNA integrants was explored at the whole genome level by analyzing RNA-seq data of Oryza sativa subsp. japonica, and utilizing sequence polymorphisms to unequivocally discriminate nuclear organelle DNA transcripts from those of bona fide cytoplasmic organelle DNA. Nuclear copies of organelle DNA that are transcribed show a spectrum of transcriptional activity but at comparatively low levels compared with the majority of other nuclear genes.
Keywords: endosymbiotic gene transfer
nuclear integrants of organellar DNA
open chromatin
Rights: © The Author(s) 2014. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (, which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited.
DOI: 10.1093/gbe/evu111
Appears in Collections:Aurora harvest 3
Molecular and Biomedical Science publications

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