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https://hdl.handle.net/2440/53171
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Type: | Journal article |
Title: | A quantitative real-time PCR method for absolute telomere length |
Author: | O'Callaghan, N. Dhillon, V. Thomas, P. Fenech, M. |
Citation: | BioTechniques: the journal of laboratory technology for bioresearch, 2008; 44(6):807-809 |
Publisher: | Eaton Publishing Co |
Issue Date: | 2008 |
ISSN: | 0736-6205 1940-9818 |
Statement of Responsibility: | Nathan J. O'Callaghan, Varinderpal S. Dhillon, Philip Thomas, Michael Fenech |
Abstract: | Telomere shortening is an important risk factor for cancer and accelerated aging. Here we describe the development of a simple and reproducible method to measure absolute telomere length. Based on Cawthon's quantitative real-time PCR (qRT-PCR) assay, our method uses an oligomer standard that can be used to generate absolute telomere length values rather than relative quantification. We demonstrate a strong correlation between this improved method and the “gold standard” of telomere length measurement—terminal restriction fragment analysis (TRF) by Southern hybridization. The capability to generate absolute telomere length values should allow a more direct comparison of results between experiments within and between laboratories. |
Keywords: | Telomere Specimen Handling Sensitivity and Specificity Reverse Transcriptase Polymerase Chain Reaction |
DOI: | 10.2144/000112761 |
Published version: | http://dx.doi.org/10.2144/000112761 |
Appears in Collections: | Aurora harvest Medicine publications |
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