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https://hdl.handle.net/2440/55436
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Type: | Journal article |
Title: | Dissection of the AtNRT2.1:AtNRT2.2 inducible high-affinity nitrate transporter gene cluster |
Author: | Li, W. Wang, Y. Okamoto, M. Crawford, N. Siddiqi, M. Glass, A. |
Citation: | Plant Physiology, 2007; 143(1):425-433 |
Publisher: | Amer Soc Plant Physiologists |
Issue Date: | 2007 |
ISSN: | 0032-0889 1532-2548 |
Statement of Responsibility: | Wenbin Li, Ye Wang, Mamoru Okamoto, Nigel M. Crawford, M. Yaeesh Siddiqi and Anthony D.M. Glass |
Abstract: | Using a new Arabidopsis (Arabidopsis thaliana) mutant (Atnrt2.1-nrt2.2) we confirm that concomitant disruption of NRT2.1 and NRT2.2 reduces inducible high-affinity transport system (IHATS) by up to 80%, whereas the constitutive high-affinity transport system (CHATS) was reduced by 30%. Nitrate influx via the low-affinity transport system (LATS) was unaffected. Shoot-to-root ratios were significantly reduced compared to wild-type plants, the major effect being upon shoot growth. In another mutant uniquely disrupted in NRT2.1 (Atnrt2.1), IHATS was reduced by up to 72%, whereas neither the CHATS nor the LATS fluxes were significantly reduced. Disruption of NRT2.1 in Atnrt2.1 caused a consistent and significant reduction of shoot-to-root ratios. IHATS influx and shoot-to-root ratios were restored to wild-type values when Atnrt2.1-nrt2.2 was transformed with a NRT2.1 cDNA isolated from Arabidopsis. Disruption of NRT2.2 in Atnrt2.2 reduced IHATS by 19% and this reduction was statistically significant only at 6 h after resupply of nitrate to nitrogen-deprived plants. Atnrt2.2 showed no significant reduction of CHATS, LATS, or shoot-to-root ratios. These results define NRT2.1 as the major contributor to IHATS. Nevertheless, when maintained on agar containing 0.25 mM KNO3 as the sole nitrogen source, Atnrt2.1-nrt2.2 consistently exhibited greater stress and growth reduction than Atnrt2.1. Evidence from real-time PCR revealed that NRT2.2 transcript abundance was increased almost 3-fold in Atnrt2.1. These findings suggest that NRT2.2 normally makes only a small contribution to IHATS, but when NRT2.1 is lost, this contribution increases, resulting in a partial compensation. |
Keywords: | Arabidopsis Plant Shoots Plant Roots Nitrates Agar Anion Transport Proteins Arabidopsis Proteins RNA, Messenger Mutagenesis, Insertional Polymerase Chain Reaction Biological Transport Mutation Multigene Family Molecular Sequence Data |
DOI: | 10.1104/pp.106.091223 |
Description (link): | http://dx.doi.org/10.1104/pp.106.091223 |
Published version: | http://dx.doi.org/10.1104/pp.106.091223 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 5 |
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