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https://hdl.handle.net/2440/55907
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Type: | Journal article |
Title: | Inhibition of restriction enzyme's DNA sequence recognition by PUVA treatment |
Author: | Hanawa, F. Okamoto, M. Towers, G. |
Citation: | Nucleic Acids Research, 2003; (sup 2001)(3):297-298 |
Publisher: | Oxford Univ Press |
Issue Date: | 2003 |
ISSN: | 0305-1048 1746-8272 |
Statement of Responsibility: | Fujinori Hanawa, Mamoru Okamoto and G.H. Neil Towers |
Abstract: | Applying various restriction enzymes on a specially designed 1.5 kb DNA fragment revealed that the inhibitory effects of PUVA treatment on restriction endonuclease activities are caused by recognition inhibition. In this study, Restriction enzymes which have a 5'-TpA sequence at the cleaving site (Kpn I, Xba I, Pme I, and Dra I), and non-cleaving site (Pac I) in recognition sites, or have two 5'-TpA sequences at the recognition site and a non-specific sequence between recognition and cleaving site (BciV I) were inhibited by PUVA treatment. Most of the other restriction enzymes used in this study which do not have a 5'-TpA sequence at their restriction site were not inhibited by PUVA treatment, although a 5'-TpA sequence is located adjacent (Sma I) or very close (BamH I, Sac I and Pst I) to the recognition and cleaving site for them. |
Keywords: | DNA Restriction Enzymes DNA PUVA Therapy Base Sequence Substrate Specificity |
Rights: | © 2003 Oxford University Press |
DOI: | 10.1093/nass/3.1.297 |
Description (link): | http://www.scopus.com/record/display.url?eid=2-s2.0-0142181130&view=basic&origin=inward&txGid=FbRdBUmXNO_mMN7PEPQMtws%3a8 |
Published version: | http://dx.doi.org/10.1093/nass/3.1.297 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 5 |
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