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https://hdl.handle.net/2440/65896
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dc.contributor.author | Selth, L. | - |
dc.contributor.author | Lorch, Y. | - |
dc.contributor.author | Ocampo-Hafalla, M. | - |
dc.contributor.author | Mitter, R. | - |
dc.contributor.author | Shales, M. | - |
dc.contributor.author | Krogan, N. | - |
dc.contributor.author | Kornberg, R. | - |
dc.contributor.author | Svejstrup, J. | - |
dc.date.issued | 2009 | - |
dc.identifier.citation | Molecular and Cellular Biology, 2009; 29(15):4220-4234 | - |
dc.identifier.issn | 0270-7306 | - |
dc.identifier.issn | 1098-5549 | - |
dc.identifier.uri | http://hdl.handle.net/2440/65896 | - |
dc.description.abstract | The histone chaperone Vps75 forms a complex with, and stimulates the activity of, the histone acetyltransferase Rtt109. However, Vps75 can also be isolated on its own and might therefore possess Rtt109-independent functions. Analysis of epistatic miniarray profiles showed that VPS75 genetically interacts with factors involved in transcription regulation whereas RTT109 clusters with genes linked to DNA replication/repair. Additional genetic and biochemical experiments revealed a close relationship between Vps75 and RNA polymerase II. Furthermore, Vps75 is recruited to activated genes in an Rtt109-independent manner, and its genome-wide association with genes correlates with transcription rate. Expression microarray analysis identified a number of genes whose normal expression depends on VPS75. Interestingly, histone H2B dynamics at some of these genes are consistent with a role for Vps75 in histone H2A/H2B eviction/deposition during transcription. Indeed, reconstitution of nucleosome disassembly using the ATP-dependent chromatin remodeler Rsc and Vps75 revealed that these proteins can cooperate to remove H2A/H2B dimers from nucleosomes. These results indicate a role for Vps75 in nucleosome dynamics during transcription, and importantly, this function appears to be largely independent of Rtt109. | - |
dc.description.statementofresponsibility | Luke A. Selth, Yahli Lorch, Maria T. Ocampo-Hafalla, Richard Mitter, Michael Shales, Nevan J. Krogan, Roger D. Kornberg, and Jesper Q. Svejstrup | - |
dc.language.iso | en | - |
dc.publisher | Amer Soc Microbiology | - |
dc.rights | Copyright © 2009, American Society for Microbiology. All Rights Reserved. | - |
dc.source.uri | http://dx.doi.org/10.1128/mcb.01882-08 | - |
dc.subject | Nucleosomes | - |
dc.subject | Saccharomyces cerevisiae | - |
dc.subject | RNA Polymerase II | - |
dc.subject | Lysine | - |
dc.subject | Saccharomyces cerevisiae Proteins | - |
dc.subject | Molecular Chaperones | - |
dc.subject | Histones | - |
dc.subject | Oligonucleotide Array Sequence Analysis | - |
dc.subject | Cluster Analysis | - |
dc.subject | Chromatin Immunoprecipitation | - |
dc.subject | Gene Expression Profiling | - |
dc.subject | Transcription, Genetic | - |
dc.subject | Binding Sites | - |
dc.subject | Protein Binding | - |
dc.subject | Acetylation | - |
dc.subject | Mutation | - |
dc.subject | Genome, Fungal | - |
dc.subject | Histone Acetyltransferases | - |
dc.title | An Rtt109-independent role for Vps75 in transcription-associated nucleosome dynamics | - |
dc.type | Journal article | - |
dc.identifier.doi | 10.1128/MCB.01882-08 | - |
pubs.publication-status | Published | - |
dc.identifier.orcid | Selth, L. [0000-0002-4686-1418] | - |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest |
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