Microbiology and Immunology

Permanent URI for this community

https://hdl.handle.net/2440/10830

Browse

Browsing Microbiology and Immunology by Author "Akoum, A."

Now showing 1 - 2 of 2
  • No Thumbnail Available
    ItemMetadata only
    Elevated concentration and biologic activity of monocyte chemotactic protein-1 levels in the peritoneal fluid of patients with endometriosis
    (Elsevier BV, 1996) Akoum, A.; Lemay, A.; McColl, S.; Turcot-Tremblay, L.; Maheux, R.

    Objective

    To estimate the concentration and the biologic activity of monocyte chemotactic protein-1 (MCP-1) in the peritoneal fluid (PF) of women with and without endometriosis.

    Design

    A case control study was conducted.

    Setting

    Gynecology clinic and Laboratories of endocrinology of reproduction and immunology.

    Patients

    Women presenting for infertility, pelvic pain, or tubal ligation in which endometriosis was diagnosed at laparoscopy (n = 36) and normal fertile controls presenting for tubal ligation (n = 21).

    Interventions

    Collection of PF via laparoscopy.

    Main outcome measures

    Determination of PF concentrations of MCP-1 by an ELISA and evaluation of its monocyte chemotactic activity using a human hystiocytic cell line (U937). RESULTS. The concentration of MCP-1 (median, range of values) was increased in the PF of endometriosis patients (283, 0 to 1,930 pg/mL; conversion factor to SI unit, 0.155) compared with the control group (140, 0 to 435 pg/mL). The most significant elevation of MCP-1 levels was found in the stage II of the disease (371, 200 to 1,930 pg/mL). An increased chemotactic activity for monocytes (mean number of migrating cells/mm2 +/- SD) also was found in stages I (1,460 +/- 312) and II (1,541 +/- 336) of the disease when compared with fertile controls (393 +/- 56). Forty percent to 53% of this activity was inhibited in the presence of an antibody specific to MCP-1.

    Conclusions

    These observations are consistent with previous data indicating increased leukocyte chemotaxis in the PF of patients with endometriosis and suggest that MCP-1 may play a relevant role in the peritoneal inflammatory reaction associated with the disease.
  • No Thumbnail Available
    ItemMetadata only
    Increased monocyte chemotactic protein-1 level and activity in the peripheral blodd of women with endometriosis
    (Elsevier BV, 1996) Akoum, A.; Lemay, A.; McColl, S.; Paradis, I.; Maheux, R.

    Objective

    Our purpose was to evaluate monocyte chemotactic protein-1 in the peripheral blood of women with and without endometriosis.

    Study design

    Fifty-seven patients with endometriosis at laparoscopy done for infertility and pelvic pain were compared with 44 fertile women with no evidence of endometriosis at tubal ligation by laparoscopy. Monocyte chemotactic protein-1 concentration in the plasma was determined by enzyme-linked immunosorbent assay and its biologic activity was evaluated by measuring monocyte chemotaxis with use of a human histiocytic cell line (U937).

    Results

    Monocyte chemotactic protein-1 concentrations (median and range of values) found in the plasma were higher in patients with endometriosis (163, 0 to 788 pg/ml) than in normal controls (0, 0 to 355 pg/ml). This elevation was significant only in the minimal stage of endometriosis (revised American Fertility Society stage I). However, increased chemotactic activity (mean number of migrating cells/mm2 +/- SEM) was found in the stages I (1240 +/- 141), II (519 +/- 30), and III-IV (523 +/- 23) of the disease compared with normal controls (205 +/- 20). A total of 35% to 44% of this activity was inhibited in the presence of an antibody specific to monocyte chemotactic protein-1.

    Conclusion

    Endometriosis is associated with increased level and activity of monocyte chemotactic protein-1 in the peripheral blood. The elevation and activation of this cytokine could play a relevant role in the immunoinflammatory process associated with the disease.