Wine Science

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Browsing Wine Science by Author "Alberts, E."

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    Comparison of ELISA and RT-PCR for the detection of PNRSV and PDV in Australian almond trees
    (Centre International de Hautes Etudes Agronomiques Méditerranénnes, 2003) Mekuria, G.; Ramesh, S.; Alberts, E.; Bertozzi, T.; Wirthensohn, M.; Collins, G.; Sedgley, M.; GREMPA Meeting on Almonds and Pistachios (13th : 2003 : Mirandela, Portugal); Oliveira, M.; Cordeiro, V.
    This three-year study compared the use of ELISA and RT-PCR for identifying Prunus necrotic ringspot virus (PNRSV) and prune dwarf virus (PDV) on 175 leaf samples taken from trees maintained as a budwood repository for almond growers. The primers used for RT-PCR were based on DNA sequences that code for coat protein, and both viruses could be detected in the same reaction. For PNRSV, both ELISA and RT-PCR produced similar results, although RT-PCR was more consistent, and had the added advantage that plant material could be tested at any time throughout the growing season. For PDV, virus particles were not detected by ELISA, but were detected in low titre using a nested PCR technique. RT-PCR is used routinely now to index progeny developed each year by the Australian almond improvement program in place of both graft incompatibility using woody indicator species and ELISA.
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    Detection of Prunus necrotic ringspot virus in almond: effect of sampling time on the efficiency of serological and biological indexing methodologies
    (C S I R O Publishing, 2002) Bertozzi, T.; Alberts, E.; Sedgley, M.
    The reliability of enzyme-linked immunosorbent assay (ELISA) and indexing methodologies for the detection of Prunus necrotic ringspot virus (PNRSV) in almond was assessed under local conditions. Thirteen field-grown almond trees were sampled fortnightly throughout the growing season. Petal and leaf homogenates were used for ELISA and to inoculate herbaceous indicator species, while buds collected from October onwards were budded to woody indicators. ELISA reliably detected PNRSV in petals and young leaves from bud-break until the cessation of stem elongation. While herbaceous indexing was not as reliable as ELISA, woody indicators could differentiate positive from negative samples reliably throughout the testing period. However, for mass screening of foundation plantings, nursery stock and elite germplasm, woody indexing is too costly and slow to give results. The use of ELISA can overcome these limitations but the timing of sample collection is critical. Petal or leaf tissue collected early in the season will yield the most reliable results.
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    Virus screening for Australian almonds
    (IAMZ-CIHEAM, 2004) Wirthensohn, M.; Ramesh, S.; Bertozzi, T.; Alberts, E.; Mekuria, G.; Bennett, C.; Collins, G.; Sedgley, M.
    As part of the Australian almond breeding program, work is conducted on breeding parents and progeny to screen for viral pathogens. Transmission of virus of up to 23% has been recorded when infected parents are used. As a service to industry the University also test the Monash almond budwood facility.