Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/100246
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Type: Journal article
Title: High content, multi-parameter analyses in buccal cells to identify Alzheimer's disease
Author: Francois, M.
Fenech, M.
Thomas, P.
Hor, M.
Rembach, A.
Martins, R.
Rainey-Smith, S.
Masters, C.
Ames, D.
Rowe, C.
Macaulay, S.
Hill, A.
Leifert, W.
Citation: Current Alzheimer Research, 2016; 13(7):787-799
Publisher: Bentham Science Publishers
Issue Date: 2016
ISSN: 1567-2050
1875-5828
Statement of
Responsibility: 
Maxime François, Michael F. Fenech, Philip Thomas, Maryam Hor, Alan Rembach, Ralph N. Martins, Stephanie R. Rainey-Smith, Colin L. Masters, David Ames, Christopher C. Rowe, S. Lance Macaulay, Andrew F. Hill, Wayne R. Leifert
Abstract: Alzheimer's disease (AD) is a degenerative brain disorder and is the most common form of dementia. Minimally invasive approaches are required that combine biomarkers to identify individuals who are at risk of developing mild cognitive impairment (MCI) and AD, to appropriately target clinical trials for therapeutic discovery as well as lifestyle strategies aimed at prevention. Buccal mucosa cells from the Australian Imaging, Biomarkers and Lifestyle Flagship Study of Ageing cohort (n=60) were investigated for cytological markers that could be used to identify both MCI and AD individuals. Visual scoring of the buccal cytome demonstrated a significantly lower frequency of basal and karyorrhectic cells in the MCI group compared with controls. A high content, automated assay was developed using laser scanning cytometry to simultaneously measure cell types, nuclear DNA content and aneuploidy, neutral lipid content, putative Tau and amyloid-β (Aβ) in buccal cells. DNA content, aneuploidy, neutral lipids and Tau were similar in all groups. However, there was significantly lower Tau protein in both basal and karyolytic buccal cell types compared with differentiated buccal cells. Aβ, as measured by frequency of cells containing Aβ signal, as well as area and integral of Aβ signal, was significantly higher in the AD group compared with the control group. Buccal cell Aβ was correlated with mini-mental state examination (MMSE) scores (r = -0.436, P=0.001) and several blood-based biomarkers. Combining newly identified biomarkers from buccal cells with those already established may offer a potential route for more specific biomarker panels which may substantially increase the likelihood of better predictive markers for earlier diagnosis of AD.
Keywords: Alzheimer's disease; amyloid; buccal cells; DNA content; laser scanning cytometry; mild cognitive impairment; neutral lipids
Description: Full text unavailable
Rights: © 2016 Bentham Science Publishers
DOI: 10.2174/1567205013666160315112151
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