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Type: Journal article
Title: Proteomic analysis reveals the potential involvement of xylanase from Pyrenophora teres f. teres in net form net blotch disease of barley
Author: Ismail, I.
Godfrey, D.
Able, A.
Citation: Australasian Plant Pathology, 2014; 43(6):715-726
Publisher: Springer
Issue Date: 2014
ISSN: 0815-3191
Statement of
I. A. Ismail, D. Godfrey, A. J. Able
Abstract: The barley pathogen Pyrenophora teres f. teres (Ptt) produces proteinaceous toxins that contribute to the necrotic symptoms observed during net form net blotch (NFNB) disease. To better understand the relationship between these toxins and virulence, a proteomics approach was used to identify proteins differentially expressed in a more virulent Ptt isolate. Three proteins were identified: an endo-1,4-β-xylanase A (PttXyn11A), a cysteine hydrolase family protein (PttCHFP1) and an unknown (but conserved) secreted protein (PttSP1). PttXyn11A was homologous to a plant cell-wall degrading enzyme but also had a predicted necrosis-inducing region on the enzyme surface. PttCHFP1 showed homology to an isochorismatase, an enzyme proposed to suppress plant defence. Xylanase activity and PttXyn11A expression were greater in more virulent isolates in vitro and during the interaction respectively, suggesting that PttXyn11A plays a role in symptom development.
Keywords: Barley net blotch; net form net blotch; virulence; proteinaceous toxins; xylanase
Rights: © Australasian Plant Pathology Society Inc. 2014
RMID: 0030015284
DOI: 10.1007/s13313-014-0314-7
Appears in Collections:Agriculture, Food and Wine publications

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