Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/102252
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Type: Journal article
Title: Enzyme-assisted extraction of carbohydrates from the brown alga Ecklonia radiata: effect of enzyme type, pH and buffer on sugar yield and molecular weight profiles
Author: Charoensiddhi, S.
Lorbeer, A.
Lahnstein, J.
Bulone, V.
Franco, C.
Zhang, W.
Citation: Process Biochemistry, 2016; 51(10):1503-1510
Publisher: Elsevier
Issue Date: 2016
ISSN: 1359-5113
1873-3298
Statement of
Responsibility: 
Suvimol Charoensiddhi, Andrew J. Lorbeer, Jelle Lahnstein, Vincent Bulone, Christopher M.M. Franco, Wei Zhang
Abstract: The aim of this study was to understand the carbohydrate yield, composition, and molecular weight (MW) profiles of extracts from the brown alga Ecklonia radiata prepared using an enzyme-assisted extraction approach. The most significant effect on the total sugar yield was observed when comparing the use of salt-containing buffers (7.9–23.9 g × 100 g−1 dry weight (DW)) to pH-adjusted water (23.6–27.9 g × 100 g−1 DW), with the buffers significantly reducing the extraction efficiency in all instances. The inclusion of selected enzymes had little or no impact on total sugar yield, when compared with the corresponding solvent or buffer-only extractions. Marginal differences on total sugar yield were found when using different carbohydrate hydrolytic enzymes and proteases, and when changing pH in pH-adjusted water extractions. Both enzymes and pH influence sugar composition, and significantly affect MW profile of the polysaccharide fractions but in different ways. The acidic extraction at pH 4.5 yields lower MW components. Enzyme-assisted extraction reduced the MW of the extracted polysaccharides by 20–50% compared to pH-adjusted water only extractions.
Keywords: Alginate; Carbohydrate extraction; Enzymatic hydrolysis; Fucoidan; Macroalgae
Description: Available online 15 July 2016
Rights: © 2016 Elsevier Ltd. All rights reserved.
DOI: 10.1016/j.procbio.2016.07.014
Grant ID: http://purl.org/au-research/grants/arc/LP150100225
Appears in Collections:Agriculture, Food and Wine publications
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