Please use this identifier to cite or link to this item: https://hdl.handle.net/2440/102324
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Type: Journal article
Title: The skin immune atlas: three-dimensional analysis of cutaneous leukocyte subsets by multiphoton microscopy
Author: Tong, P.
Roediger, B.
Kolesnikoff, N.
Biro, M.
Tay, S.
Jain, R.
Shaw, L.
Grimbaldeston, M.
Weninger, W.
Citation: Journal of Investigative Dermatology, 2015; 135(1):84-93
Publisher: Nature Publishing Group
Issue Date: 2015
ISSN: 0022-202X
1523-1747
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Responsibility: 
Philip L. Tong, Ben Roediger, Natasha Kolesnikoff, Maté Biro, Szun S. Tay, Rohit Jain, Lisa E. Shaw, Michele A. Grimbaldeston and Wolfgang Weninger
Abstract: Site-specific differences in skin response to pathogens and in the course of cutaneous inflammatory diseases are well appreciated. The composition and localization of cutaneous leukocytes has been studied extensively using histology and flow cytometry. However, the precise three-dimensional (3D) distribution of distinct immune cell subsets within skin at different body sites requires visualization of intact living skin. We used intravital multiphoton microscopy in transgenic reporter mice in combination with quantitative flow cytometry to generate a 3D immune cell atlas of mouse skin. The 3D location of innate and adaptive immune cells and site-specific differences in the densities of macrophages, T cells, and mast cells at four defined sites (ear, back, footpad, and tail) is presented. The combinatorial approach further demonstrates an as yet unreported age-dependent expansion of dermal gamma-delta T cells. Localization of dermal immune cells relative to anatomical structures was also determined. Although dendritic cells were dispersed homogeneously within the dermis, mast cells preferentially localized to the perivascular space. Finally, we show the functional relevance of site-specific mast cell disparities using the passive cutaneous anaphylaxis model. These approaches are applicable to assessing immune cell variations and potential functional consequences in the setting of infection, as well as the pathogenesis of inflammatory skin conditions.
Keywords: Leukocytes
Rights: © 2015 The Society for Investigative Dermatology
DOI: 10.1038/jid.2014.289
Grant ID: http://purl.org/au-research/grants/nhmrc/1066477
http://purl.org/au-research/grants/nhmrc/1047041
http://purl.org/au-research/grants/nhmrc/1011203
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