Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/102915
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Type: Journal article
Title: Isolation and plasmid characterization of carbapenemase (IMP-4) producing Salmonella enterica Typhimurium from cats
Author: Abraham, S.
O'Dea, M.
Trott, D.
Abraham, R.
Hughes, D.
Pang, S.
McKew, G.
Cheong, E.
Merlino, J.
Saputra, S.
Malik, R.
Gottlieb, T.
Citation: Scientific Reports, 2016; 6(1):35527-1-35527-7
Publisher: Nature Publishing Group
Issue Date: 2016
ISSN: 2045-2322
2045-2322
Statement of
Responsibility: 
Sam Abraham, Mark O'Dea, Darren J. Trott, Rebecca J. Abraham, David Hughes, Stanley Pang, Genevieve McKew, Elaine Y. L. Cheong, John Merlino, Sugiyono Saputra, Richard Malik, Thomas Gottlieb
Abstract: Carbapenem-resistant Enterobacteriaceae (CRE) are a pressing public health issue due to limited therapeutic options to treat such infections. CREs have been predominantly isolated from humans and environmental samples and they are rarely reported among companion animals. In this study we report on the isolation and plasmid characterization of carbapenemase (IMP-4) producing Salmonella enterica Typhimurium from a companion animal. Carbapenemase-producing S. enterica Typhimurium carrying blaIMP-4 was identified from a systemically unwell (index) cat and three additional cats at an animal shelter. All isolates were identical and belonged to ST19. Genome sequencing revealed the acquisition of a multidrug-resistant IncHI2 plasmid (pIMP4-SEM1) that encoded resistance to nine antimicrobial classes including carbapenems and carried the blaIMP-4-qacG-aacA4-catB3 cassette array. The plasmid also encoded resistance to arsenic (MIC-150 mM). Comparative analysis revealed that the plasmid pIMP4-SEM1 showed greatest similarity to two blaIMP-8 carrying IncHI2 plasmids from Enterobacter spp. isolated from humans in China. This is the first report of CRE carrying a blaIMP-4 gene causing a clinical infection in a companion animal, with presumed nosocomial spread. This study illustrates the broader community risk entailed in escalating CRE transmission within a zoonotic species such as Salmonella, and in a cycle that encompasses humans, animals and the environment.
Keywords: Animals; Cats; Humans; Salmonella typhimurium; beta-Lactamases; Bacterial Proteins; Drug Resistance, Multiple, Bacterial; Plasmids; China
Rights: © The Author(s) 2016. This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
RMID: 0030057142
DOI: 10.1038/srep35527
Appears in Collections:Animal and Veterinary Sciences publications

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