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Type: Journal article
Title: Blood-based protein biomarker panel for the detection of colorectal cancer
Author: Fung, K.
Tabor, B.
Buckley, M.
Priebe, I.
Purins, L.
Pompeia, C.
Brierley, G.
Lockett, T.
Gibbs, P.
Tie, J.
McMurrick, P.
Moore, J.
Ruszkiewicz, A.
Nice, E.
Adams, T.
Burgess, A.
Cosgrove, L.
Citation: PLoS One, 2015; 10(3):e0120425-1-e0120425-11
Publisher: Public Library of Science
Issue Date: 2015
ISSN: 1932-6203
Editor: Hoheisel, J.
Statement of
Kim Y. C. Fung, Bruce Tabor, Michael J. Buckley, Ilka K. Priebe, Leanne Purins, Celine Pompeia, Gemma V. Brierley, Trevor Lockett, Peter Gibbs, Jeanne Tie, Paul McMurrick, James Moore, Andrew Ruszkiewicz, Edouard Nice, Timothy E. Adams, Antony Burgess, Leah J. Cosgrove
Abstract: Background: The majority of colorectal cancer (CRC) cases are preventable by early detection and removal of precancerous polyps. Even though CRC is the second most common internal cancer in Australia, only 30 per cent of the population considered to have risk factors participate in stool-based test screening programs. Evidence indicates a robust, blood-based, diagnostic assay would increase screening compliance. A number of potential diagnostic blood-based protein biomarkers for CRC have been reported, but all lack sensitivity or specificity for use as a stand-alone diagnostic. The aim of this study was to identify and validate a panel of protein-based biomarkers in independent cohorts that could be translated to a reliable, non-invasive blood-based screening test. Principal Findings: In two independent cohorts (n = 145 and n = 197), we evaluated seven single biomarkers in serum of CRC patients and age/gender matched controls that showed a significant difference between controls and CRC, but individually lack the sensitivity for diagnostic application. Using logistic regression strategies, we identified a panel of three biomarkers that discriminated between controls and CRC with 73% sensitivity at 95% specificity, when applied to either of the two cohorts. This panel comprised of Insulin like growth factor binding protein 2 (IGFBP2), Dickkopf-3 (DKK3), and Pyruvate kinase M2(PKM2). Conclusions: Due to the heterogeneous nature of CRC, a single biomarker is unlikely to have sufficient sensitivity or specificity for use as a stand-alone diagnostic screening test and a panel of markers may be more effective. We have identified a 3 biomarker panel that has higher sensitivity and specificity for early stage (Stage I and -II) disease than the faecal occult blood test, raising the possibility for its use as a non-invasive blood diagnostic or screening test.
Keywords: Humans
Colorectal Neoplasms
Thyroid Hormones
Intercellular Signaling Peptides and Proteins
Adaptor Proteins, Signal Transducing
Carrier Proteins
Insulin-Like Growth Factor Binding Protein 2
Membrane Proteins
Case-Control Studies
Aged, 80 and over
Middle Aged
Biomarkers, Tumor
Rights: © 2015 Fung et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
DOI: 10.1371/journal.pone.0120425
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