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Type: Journal article
Title: A novel embryo culture media supplement that improves pregnancy rates in mice
Author: Highet, A.
Bianco-Miotto, T.
Pringle, K.
Peura, A.
Bent, S.
Zhang, J.
Nottle, M.
Thompson, J.
Roberts, C.
Citation: Reproduction, 2017; 153(3):327-340
Publisher: Society for Reproduction and Fertility
Issue Date: 2017
ISSN: 1470-1626
Statement of
A R Highet, T Bianco-Miotto, K G Pringle, A Peura, S Bent, J Zhang, M B Nottle, J G Thompson and C T Roberts
Abstract: The preimplantation embryo in vivo is exposed to numerous growth factors in the female reproductive tract, which are not recapitulated in embryo culture media in vitro The IGF2 and plasminogen activator systems facilitate blastocyst development. We hypothesized that the addition of IGF2 in combination with urokinase plasminogen activator (uPA) and plasminogen could improve rates of blastocyst hatching and implantation in mice. B6BcF1 and CBAB6F2 mouse embryos were divided into one of four supplemented culture media treatment groups: (1) control (media only); (2) 12.5 nM IGF2; (3) 10 µg/mL uPA and 5 µg/mL plasminogen; or (4) a combination of IGF2, uPA and plasminogen treatments. Embryo development to blastocyst stage and hatching were assessed before transfer to pseudopregnant recipient females and implantation, pregnancy rates and postnatal growth were assessed. After 90.5 h of culture, IGF2 + U + P treatment increased the percentage of B6BcF1 embryos that were hatching/hatched and percentage developing to blastocyst stage compared with controls (P < 0.02). Following B6BcF1 embryo transfer, IGF2 + U + P treatment increased implantation sites at day 8 of pregnancy compared with controls (P < 0.05). Replication in the CBAB6F2 mouse strain showed significant improvements in pregnancy rates at days 8 and 18 but not in blastocyst development. No adverse effects were seen on gestational age, litter size or birthweight, or the reproductive capacity of offspring of IGF2 + U + P treated embryos. For embryos susceptible to detrimental effects of in vitro culture, IGF2, uPA and plasminogen supplementation of culture media can improve pregnancy success, but the effect of treatment is dependent on the mouse strain.
Keywords: Blastocyst; Animals; Mice, Inbred C57BL; Mice; Plasminogen; Insulin-Like Growth Factor II; Culture Media; Embryo Transfer; Fertilization in Vitro; Embryo Culture Techniques; Pregnancy Rate; Embryonic Development; Embryo Implantation; Pregnancy; Female; Urokinase-Type Plasminogen Activator
Rights: © 2017 Society for Reproduction and Fertility
RMID: 0030064405
DOI: 10.1530/REP-16-0517
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Appears in Collections:Paediatrics publications

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