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Type: Journal article
Title: Multifunctional regulation of angiogenesis by high-density lipoproteins
Author: Prosser, H.
Tan, J.
Dunn, L.
Patel, S.
Vanags, L.
Bao, S.
Ng, M.
Bursill, C.
Citation: Cardiovascular Research, 2014; 101(1):145-154
Publisher: Oxford University Press
Issue Date: 2014
ISSN: 0008-6363
Statement of
Hamish C.G. Prosser, Joanne T.M. Tan, Louise L. Dunn, Sanjay Patel, Laura Z. Vanags, Shisan Bao, Martin K.C. Ng, and Christina A. Bursill
Abstract: Aims: High-density lipoproteins (HDL) exert striking anti-inflammatory effects and emerging evidence suggests that they may augment ischaemia-mediated neovascularization. We sought to determine whether HDL conditionally regulates angiogenesis, depending on the pathophysiological context by (i) inhibiting inflammation-induced angiogenesis, but also; (ii) enhancing ischaemia-mediated angiogenesis. Methods and results: Intravenously delivered apolipoprotein (apo) A-I attenuated neovascularization in the murine femoral collar model of inflammation-induced angiogenesis, compared with phosphate-buffered saline infused C57BL6/J mice (58%), P < 0.05. Conversely, apoA-I delivery augmented neovessel formation (75%) and enhanced blood perfusion (45%) in the murine hindlimb ischaemia model, P < 0.05. Reconstituted HDL (rHDL) was tested on key angiogenic cell functions in vitro. rHDL inhibited human coronary artery endothelial cell migration (37.9 and 76.9%), proliferation (15.7 and 40.4%), and tubulogenesis on matrigel (52 and 98.7%) when exposed to two inflammatory stimuli: tumour necrosis factor-α (TNF-α) and macrophage-conditioned media (MCM). In contrast, rHDL significantly augmented hypoxia-stimulated migration (36.9%), proliferation (135%), and tubulogenesis (22.9%), P < 0.05. Western blot and RT–PCR analyses revealed that these divergent actions of rHDL were associated with conditional regulation of hypoxia-inducible factor-1α (HIF-1α), vascular endothelial growth factor (VEGF) and VEGF receptor 2, which were attenuated in response to TNF-α (40.4, 41.0, and 33.2%) and MCM (72.5, 30.7, and 69.5%), but augmented by rHDL in hypoxia (39.8, 152.6, and 15.7%%), all P < 0.05. Conclusion: HDL differentially regulates angiogenesis dependent upon the pathophysiological setting, characterized by suppression of inflammation-associated angiogenesis, and conversely, by the enhancement of hypoxia-mediated angiogenesis. This has significant implications for therapeutic modulation of neovascularization.
Keywords: Cells, Cultured; Animals; Mice, Inbred C57BL; Humans; Neovascularization, Pathologic; Vascular Endothelial Growth Factor Receptor-2; Lipoproteins, HDL; Vascular Endothelial Growth Factor A; Apolipoprotein A-I; Random Allocation; Neovascularization, Physiologic; Male; Hypoxia-Inducible Factor 1, alpha Subunit
Rights: All rights reserved. © The Author 2013. For permissions please email:
RMID: 0030067653
DOI: 10.1093/cvr/cvt234
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Appears in Collections:Medicine publications

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