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Type: Journal article
Title: Effects of resveratrol supplementation on methotrexate chemotherapy-induced bone loss
Author: Lee, A.
Shandala, T.
Soo, P.
Su, Y.
King, T.
Chen, K.
Howe, P.
Xian, C.
Citation: Nutrients, 2017; 9(3):255-1-255-15
Publisher: MDPI
Issue Date: 2017
ISSN: 2072-6643
Statement of
Alice M.C. Lee, Tetyana Shandala, Pei Pei Soo, Yu-Wen Su, Tristan J. King, Ke-Ming Chen, Peter R. Howe and Cory J. Xian
Abstract: Intensive cancer chemotherapy is known to cause bone defects, which currently lack treatments. This study investigated the effects of polyphenol resveratrol (RES) in preventing bone defects in rats caused by methotrexate (MTX), a commonly used antimetabolite in childhood oncology. Young rats received five daily MTX injections at 0.75 mg/kg/day. RES was orally gavaged daily for seven days prior to, and during, five-day MTX administration. MTX reduced growth plate thickness, primary spongiosa height, trabecular bone volume, increased marrow adipocyte density, and increased mRNA expression of the osteogenic, adipogenic, and osteoclastogenic factors in the tibial bone. RES at 10 mg/kg was found not to affect bone health in normal rats, but to aggravate the bone damage in MTX-treated rats. However, RES supplementation at 1 mg/kg preserved the growth plate, primary spongiosa, bone volume, and lowered the adipocyte density. It maintained expression of genes involved in osteogenesis and decreased expression of adipogenic and osteoclastogenic factors. RES suppressed osteoclast formation ex vivo of bone marrow cells from the treated rats. These data suggest that MTX can enhance osteoclast and adipocyte formation and cause bone loss, and that RES supplementation at 1 mg/kg may potentially prevent these bone defects.
Keywords: Resveratrol; cancer chemotherapy; methotrexate; bone loss; bone marrow adiposity; bone growth arrest; growth plate; osteoclasts; osteoblasts; adipocytes
Rights: © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (
RMID: 0030071137
DOI: 10.3390/nu9030255
Appears in Collections:Medicine publications

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