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Type: Journal article
Title: The polyketide synthase OsPKS2 is essential for pollen exine and Ubisch body patterning in rice
Author: Zhu, X.
Yu, J.
Shi, J.
Tohge, T.
Fernie, A.
Meir, S.
Aharoni, A.
Xu, D.
Zhang, D.
Liang, W.
Citation: Journal of Integrative Plant Biology, 2017; 59(9):612-628
Publisher: Wiley
Issue Date: 2017
ISSN: 1672-9072
Statement of
Xiaolei Zhu, Jing Yu, Jianxin Shi, Takayuki Tohge, Alisdair R. Fernie, Sagit Meir, Asaph Aharoni, Dawei Xu, Dabing Zhang and Wanqi Liang
Abstract: Lipid and phenolic metabolism are important for pollen exine formation. In Arabidopsis, polyketide synthases (PKSs) are essential for both sporopollenin biosynthesis and exine formation. Here, we characterized the role of a polyketide synthase (OsPKS2) in male reproduction of rice (Oryza sativa). Recombinant OsPKS2 catalyzed the condensation of fatty acyl-CoA with malonyl-CoA to generate triketide and tetraketide α-pyrones, the main components of pollen exine. Indeed, the ospks2 mutant had defective exine patterning and was male sterile. However, the mutant showed no significant reduction in sporopollenin accumulation. Compared with the WT (wild type), ospks2 displayed unconfined and amorphous tectum and nexine layers in the exine, and less organized Ubisch bodies. Like the pksb/lap5 mutant of the Arabidopsis ortholog, ospks2 showed broad alterations in the profiles of anther-related phenolic compounds. However, unlike pksb/lap5, in which most detected phenolics were substantially decreased, ospks2 accumulated higher levels of phenolics. Based on these results and our observation that OsPKS2 is unable to fully restore the exine defects in the pksb/lap5, we propose that PKS proteins have functionally diversified during evolution. Collectively, our results suggest that PKSs represent a conserved and diversified biochemical pathway for anther and pollen development in higher plants.
Keywords: Polyketide Synthases
Rights: © 2017 Institute of Botany, Chinese Academy of Sciences
DOI: 10.1111/jipb.12574
Appears in Collections:Agriculture, Food and Wine publications
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