Please use this identifier to cite or link to this item:
Scopus Web of ScienceĀ® Altmetric
Type: Journal article
Title: Identification of Novel Alternatively Spliced Pyruvate Carboxylase mRNAs with Divergent 5' -Untranslated Regions Which Are Expressed in a Tissue-Specific Manner
Author: Jitrapakdee, S.
Walker, M.
Wallace, J.
Citation: Biochemical and Biophysical Research Communications, 1996; 223(3):695-700
Issue Date: 1996
ISSN: 0006-291X
Abstract: We have identified and characterized multiple mRNA transcripts of rat and human pyruvate carboxylases [EC] using rapid amplification of cDNA 5' ends-polymerase chain reaction (RACE-PCR). Five alternative forms of rat pyruvate carboxylase cDNAs have been identified in liver, kidney, brain, and adipose tissue and these are expressed in a tissue-specific manner. Two alternative forms of human pyruvate carboxylase cDNA have also been identified in liver. These pyruvate carboxylase cDNAs have a common coding region but differ in their 5' untranslated regions (5'UTRs), suggesting that they are generated by alternative splicing of the primary transcript. Southern blot analysis of restriction enzyme digested rat genomic DNA revealed that pyruvate carboxylase is encoded by a single copy gene.
Keywords: Kidney
Pyruvate Carboxylase
Recombinant Proteins
RNA, Messenger
DNA Primers
Cloning, Molecular
Random Amplified Polymorphic DNA Technique
Protein Biosynthesis
Alternative Splicing
Base Sequence
Regulatory Sequences, Nucleic Acid
Sequence Homology, Nucleic Acid
Molecular Sequence Data
DOI: 10.1006/bbrc.1996.0958
Published version:
Appears in Collections:Aurora harvest 2
Biochemistry publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.