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https://hdl.handle.net/2440/114127
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Type: | Journal article |
Title: | Identification and characterisation of a previously unknown drought tolerance-associated microRNA in barley |
Author: | Zhou, H. Hussain, S.S. Hackenberg, M. Bazanova, N. Eini Gandomani, O. Li, J. Gustafson, P. Shi, B. |
Citation: | The Plant Journal, 2018; 95(1):138-149 |
Publisher: | Wiley |
Issue Date: | 2018 |
ISSN: | 0960-7412 1365-313X |
Statement of Responsibility: | Hui Zhou, Syed S. Hussain, Michael Hackenberg, Natalia Bazanova, Omid Eini, Jie Li, Perry Gustafson, Bujun Shi |
Abstract: | Drought is the most serious abiotic stress, and causes crop losses on a worldwide scale. The present study identified a previously unknown microRNA (designated as hvu-miRX) of 21 nucleotides (nt) in length in barley. Its precursor (designated pre-miRX) and primary transcript (designated pri-miRX) were also identified, with lengths of 73 and 559 nt, respectively. The identified upstream sequence of pri-miRX contained both the TATA box and the CAAT box, which are both required for initiation of transcription. Transient promoter activation assays showed that the core promoter region of pri-miRX ranged 500 nt from the transcription start site. In transgenic barley overexpression of the wheat DREB3 transcription factor (TaDREB3) caused hvu-miRX to be highly expressed as compared with the same miRNA in non-transgenic barley. However, the high expression was not directly associated with TaDREB3. Genomic analysis revealed that the hvu-miRX gene was a single copy located on the short arm of chromosome 2 and appeared to be only conserved in Triticeae, but not in other plant species. Notably, transgenic barley that overexpressed hvu-miRX showed drought tolerance. Degradome library analysis and other tests showed that hvu-miRX targeted various genes including transcription factors via the cleavage mode. Our data provides an excellent opportunity to develop drought stress tolerant cereals using hvu-miRX. |
Keywords: | Drought tolerance miRNA precursors miRNA promoter novel miRNA transgenic barley |
Rights: | © 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd. |
DOI: | 10.1111/tpj.13938 |
Grant ID: | ARC |
Published version: | http://dx.doi.org/10.1111/tpj.13938 |
Appears in Collections: | Agriculture, Food and Wine publications Aurora harvest 3 |
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