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Type: Journal article
Title: Analysis of plastid DNA-like sequences within the nuclear genomes of higher plants.
Author: Ayliffe, M.
Scott, N.
Timmis, J.
Citation: Molecular Biology and Evolution, 1998; 15(6):738-745
Issue Date: 1998
ISSN: 0737-4038
Abstract: A wide-ranging examination of plastid (pt)DNA sequence homologies within higher plant nuclear genomes (promiscuous DNA) was undertaken. Digestion with methylation-sensitive restriction enzymes and Southern analysis was used to distinguish plastid and nuclear DNA in order to assess the extent of variability of promiscuous sequences within and between plant species. Some species, such as Gossypium hirsutum (cotton), Nicotiana tabacum (tobacco), and Chenopodium quinoa, showed homogenity of these sequences, while intraspecific sequence variation was observed among different cultivars of Pisum sativum (pea), Hordeum vulgare (barley), and Triticum aestivum (wheat). Hypervariability of plastid sequence homologies was identified in the nuclear genomes of Spinacea oleracea (spinach) and Beta vulgaris (beet), in which individual plants were shown to possess a unique spectrum of nuclear sequences with ptDNA homology. This hypervariability apparently extended to somatic variation in B. vulgaris. No sequences with ptDNA homology were identified by this method in the nuclear genome of Arabidopsis thaliana.
Keywords: Plastids; Plants; Arabidopsis; Chenopodiaceae; Spinacia oleracea; Peas; Gossypium; Hordeum; Triticum; Tobacco; Plants, Toxic; DNA, Plant; Species Specificity; Sequence Homology, Nucleic Acid; Genome, Plant; Edible Grain
RMID: 0030004282
DOI: 10.1093/oxfordjournals.molbev.a025977
Appears in Collections:Genetics publications

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