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Type: Journal article
Title: A molecular and evolutionary study of the β-globin gene family of the Australian marsupial Sminthopsis Crassicaudata
Other Titles: A molecular and evolutionary study of the beta-globin gene family of the Australian marsupial Sminthopsis crassicaudata
Author: Cooper, S.
Murphy, R.
Dolman, G.
Hussey, D.
Hope, R.
Citation: Molecular Biology and Evolution, 1996; 13(7):1012-1022
Issue Date: 1996
ISSN: 0737-4038
Statement of
S J Cooper, R Murphy, G Dolman, D Hussey and R M Hope
Abstract: Beta-globin gene families in eutherians (placental mammals) consist of a set of four or more developmentally regulated genes which are closely linked and, in general, arranged in the order 5'-embryonic/fetal genes-adult genes-3'. This cluster of genes is proposed to have arisen by tandem duplication of ancestral beta-globin genes, with the first duplication occurring 200 to 155 MYBP just prior to a period in mammalian evolution when eutherians and marsupials diverged from a common ancestor. In this paper we trace the evolutionary history of the beta-globin gene family back to the origins of these mammals by molecular characterization of the beta-globin gene family of the Australian marsupial Sminthopsis crassicaudata. Using Southern and restriction analysis of total genomic DNA and bacteriophage clones of beta-like globin genes, we provide evidence that just two functional beta-like globin genes exist in this marsupial, including one embryonic-expressed gene (S.c-epsilon) and one adult-expressed gene (S.c-beta), linked in the order 5'-epsilon-beta-3'. The entire DNA sequence of the adult beta-globin gene is reported and shown to be orthologous to the adult beta-globin genes of the North American marsupial Didelphis virginiana and eutherian mammals. These results, together with results from a phylogenetic analysis of mammalian beta-like globin genes, confirm the hypothesis that a two-gene cluster, containing an embryonic- and an adult-expressed beta-like globin gene, existed in the most recent common ancester of marsupials and eutherians. Northern analysis of total RNA isolated from embryos and neonatals indicates that a switch from embryonic to adult gene expression occurs at the time of birth, coinciding with the transfer of the marsupial from a uterus to a pouch environment.
Keywords: Animals
Deoxyribonuclease BamHI
Site-Specific DNA-Methyltransferase (Adenine-Specific)
DNA Probes
Blotting, Northern
Blotting, Southern
In Situ Hybridization
Restriction Mapping
Cloning, Molecular
Age Factors
Evolution, Molecular
Gene Expression Regulation, Developmental
Amino Acid Sequence
Base Sequence
Multigene Family
Molecular Sequence Data
Genetic Variation
Genetic Linkage
Rights: © 1996 Society for Molecular Biology and Evolution
DOI: 10.1093/oxfordjournals.molbev.a025651
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