Please use this identifier to cite or link to this item: http://hdl.handle.net/2440/114829
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dc.contributor.authorHickey, E.en
dc.contributor.authorWong, H.en
dc.contributor.authorKhazandi, M.en
dc.contributor.authorOgunniyi, A.en
dc.contributor.authorPetrovski, K.en
dc.contributor.authorGarg, S.en
dc.contributor.authorPage, S.en
dc.contributor.authorO'Handley, R.en
dc.contributor.authorTrott, D.en
dc.date.issued2018en
dc.identifier.citationJournal of Veterinary Pharmacology and Therapeutics, 2018; 41(5):746-754en
dc.identifier.issn0140-7783en
dc.identifier.issn1365-2885en
dc.identifier.urihttp://hdl.handle.net/2440/114829-
dc.description.abstractIncreasing reports of multidrug-resistant bacterial infections in animals has created a need for novel antimicrobial agents that do not promote cross-resistance to critically important antimicrobial classes used in human medicine. In response to the recent emergence of antimicrobial resistance in several bovine mastitis pathogens, in vitro antimicrobial susceptibility was determined for four polyether ionophores (lasalocid, monensin, narasin and salinomycin) against Staphylococcus spp. and Streptococcus spp. isolated from clinical cases. In addition, erythrocyte haemolysis and WST-1 cell proliferation assays were used to assess in vitro mammalian cell cytotoxicity and biofilm susceptibility testing was performed using the minimum biofilm eradication concentration (MBEC™) biofilm assay. Lasalocid, monensin, narasin and salinomycin exhibited bacteriostatic antimicrobial activity against all pathogens tested, including methicillin-resistant staphylococci, with MIC₉₀ values <16 μg/ml. Narasin and monensin displayed the least toxicity against mammalian cell lines and all compounds significantly reduced viable cell numbers in a Staphylococcus aureus biofilm. Based on in vitro characterization, all four ionophores offer potentially novel treatments against bovine mastitis but in vivo studies will be essential to determine whether acceptable safety and efficacy is present following intramammary administration.en
dc.description.statementofresponsibilityElizabeth E. Hickey, Hui San Wong, Manouchehr Khazandi, Abiodun D. Ogunniyi, Kiro R. Petrovski, Sanjay Garg, Stephen W. Page, Ryan O'Handley, Darren J. Trotten
dc.language.isoenen
dc.publisherWileyen
dc.rights© 2018 John Wiley & Sons Ltden
dc.subjectBiofilms; cytotoxicity; Gram-positive; Ionophores; mastitis; methicillin-resistanten
dc.titleRepurposing Ionophores as novel antimicrobial agents for the treatment of bovine mastitis caused by Gram-positive pathogensen
dc.typeJournal articleen
dc.identifier.rmid0030095347en
dc.identifier.doi10.1111/jvp.12674en
dc.relation.granthttp://purl.org/au-research/grants/arc/LP130100736en
dc.identifier.pubid433071-
pubs.library.collectionAnimal and Veterinary Sciences publicationsen
pubs.library.teamDS03en
pubs.verification-statusVerifieden
pubs.publication-statusPublisheden
dc.identifier.orcidHickey, E. [0000-0002-4862-503X]en
dc.identifier.orcidKhazandi, M. [0000-0002-9806-9959]en
dc.identifier.orcidOgunniyi, A. [0000-0001-9308-5629]en
dc.identifier.orcidPetrovski, K. [0000-0003-4016-2576]en
dc.identifier.orcidO'Handley, R. [0000-0002-6046-0688]en
dc.identifier.orcidTrott, D. [0000-0002-8297-5770]en
Appears in Collections:Animal and Veterinary Sciences publications

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