Please use this identifier to cite or link to this item:
Scopus Web of Science® Altmetric
Full metadata record
DC FieldValueLanguage
dc.contributor.authorHaines, B.-
dc.contributor.authorRathjen, P.-
dc.contributor.authorHope, R.-
dc.contributor.authorWhyatt, L.-
dc.contributor.authorHolland, M.-
dc.contributor.authorBreed, W.-
dc.identifier.citationMolecular Reproduction and Development, 1999; 52(2):174-182-
dc.descriptionArticle first published online: 4 JAN 1999-
dc.description.abstractWe have cloned a cDNA containing the entire coding sequence of a marsupial (the brushtail possum, Trichosurus vulpecula) zona pellucida protein (ZPB). The open reading frame of 1,581 nt is predicted to encode a ZPB polypeptide of 527 amino acids which contains 20 cysteine residues, 7 potential N-linked glycosylation sites, a potential N-terminal signal peptide and a potential C-terminal trans-membrane domain, preceded by a furin proteolytic processing signal. Sequence comparisons between possum ZPB and orthologous polypeptides from 7 eutherian species and from Xenopus laevis, reveal the existence of a high degree of sequence similarity, particularly in the central portion of the molecule. Cysteine residues are highly conserved, and all nine species possess potential N-terminal signal peptide sequences and C-terminal trans-membrane domains of approximately the same length. In situ hybridisation revealed that expression of ZPB was restricted to oocytes of primordial and primary follicles of adult possums; no expression was detected in the surrounding granulosa cells. The broad conservation of ZPB sequence, structure and expression over a wide range of mammalian species, revealed by our studies, makes it unlikely that these features account for the different properties of the marsupial and eutherian zona pellucidae.-
dc.rightsCopyright © 1999 Wiley-Liss, Inc.-
dc.subjectEgg Proteins-
dc.subjectMembrane Glycoproteins-
dc.subjectReceptors, Cell Surface-
dc.subjectDNA, Complementary-
dc.subjectGene Expression-
dc.subjectAmino Acid Sequence-
dc.subjectBase Sequence-
dc.subjectSequence Homology, Amino Acid-
dc.subjectMolecular Sequence Data-
dc.subjectZona Pellucida Glycoproteins-
dc.titleIsolation and characterisation of a cDNA encoding a Zona Pellucida Protein (ZPB) from the marsupial Trichosurus vulpecula (Brushtail Possum)-
dc.typeJournal article-
dc.identifier.orcidWhyatt, L. [0000-0002-4504-6534]-
dc.identifier.orcidBreed, W. [0000-0003-1739-6593]-
Appears in Collections:Aurora harvest 2
Environment Institute publications
Genetics publications

Files in This Item:
There are no files associated with this item.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.