Adelaide Research & Scholarship
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https://hdl.handle.net/2440/11536
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| Type: | Journal article |
| Title: | Recovery of poly-3-hydroxybutyrate from recombinant Escherichia coli by homogenization and centrifugation |
| Author: | Ling, Y. Williams, D. Thomas, C. Middelberg, A. |
| Citation: | Biotechnology Letters, 1997; 11(6):409-412 |
| Publisher: | CHAPMAN HALL LTD |
| Issue Date: | 1997 |
| ISSN: | 0951-208X |
| Abstract: | Poly-3-hydroxybutyrate from recombinant E. coli was recovered using homogenization and continuous centrifugation with a purity of 94%. Final protein and DNA concentrations were 1.0% w/w and 1.9% w/w, respectively, when a hypochlorite treatment was employed prior to centrifugation. High fractional cell debris removal (94%) was achieved with two centrifugation steps. |
| DOI: | 10.1023/A:1018416706294 |
| Published version: | http://dx.doi.org/10.1023/a:1018416706294 |
| Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
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