Please use this identifier to cite or link to this item:
https://hdl.handle.net/2440/11574
Citations | ||
Scopus | Web of ScienceĀ® | Altmetric |
---|---|---|
?
|
?
|
Type: | Journal article |
Title: | Comparison of the promoter proximal regions of the toxin-co-regulated tcp gene cluster in classical and El Tor strains of Vibrio cholerae O1 |
Author: | Ogierman, M. Voss, E. Meaney, C. Faast, R. Attridge, S. Manning, P. |
Citation: | Gene, 1996; 17(1):9-16 |
Publisher: | ELSEVIER SCIENCE BV |
Issue Date: | 1996 |
ISSN: | 0378-1119 1879-0038 |
Abstract: | A physical map has been constructed of the 5-kb XbaI fragment encoding the promoter proximal of region the tcp gene cluster encoding the toxin-coregulated pilus (TCP) of Vibrio cholerae. This fragment contains the major regulatory regions for TCP. Comparison of the nucleotide (nt) sequences from strains of the classical and El Tor biotypes demonstrates that the regions are essentially identical, with several notable exceptions. The intergenic regions, between tcpI and tcpP, and between tcpH and tcpA, show significant sequence divergence which may account for the biotype-related differences in TCP, since this is the location of the major promoter sequences. The C-terminal coding regions of the major pilin subunit, TcpA, also differ. Southern hybridization analyses suggest that the tcpA nt sequence is conserved within a biotype, and Western blot analysis suggests that the two forms of TcpA are antigenically different, but related. Besides tcpA, tcpB, tcpH and tcpI, the genes encoding two additional proteins, TcpP and TcpQ, but not previously defined, were also identified. TcpH and TcpI have been previously suggested to be regulatory proteins but homology data imply that TcpI is a methyl-accepting chemotaxis protein (MCP), as recently reported [Harkey et al., Infect. Immun. 62 (1994) 2669-2678], and TcpH is predicted to be a periplasmic or exported protein. TcpP is thought to be a trans-cytoplasmic membrane (CM) protein which may have a regulatory role. |
Keywords: | Fimbriae, Bacterial Vibrio cholerae Lipoproteins Bacterial Proteins Bacterial Outer Membrane Proteins Fimbriae Proteins Transcription Factors Repressor Proteins Bacterial Adhesion Virulence Species Specificity Gene Expression Regulation, Bacterial Amino Acid Sequence Base Sequence Sequence Homology Genes, Bacterial Genes, Regulator Molecular Sequence Data Promoter Regions, Genetic |
DOI: | 10.1016/0378-1119(95)00744-X |
Published version: | http://dx.doi.org/10.1016/0378-1119(95)00744-x |
Appears in Collections: | Aurora harvest 7 Microbiology and Immunology publications |
Files in This Item:
There are no files associated with this item.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.