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https://hdl.handle.net/2440/11610
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Type: | Journal article |
Title: | Molecular and Genetic Characterization of the Capsule Biosynthesis Locus Of Streptococcus pneumoniae Type 19B |
Author: | Morona, J. Morona, R. Paton, J. |
Citation: | Journal of Bacteriology, 1997; 179(15):4953-4958 |
Publisher: | AMER SOC MICROBIOLOGY |
Issue Date: | 1997 |
ISSN: | 0021-9193 1098-5530 |
Abstract: | We have previously reported the nucleotide sequence of the Streptococcus pneumoniae type 19F capsular polysaccharide synthesis locus (cps19f), which consists of 15 open reading frames (ORFs) designated cps19fA to -O. Hybridization analysis indicated that close homologs for cps19fA to -H and cps19fK to -O were found in type 19B, but there were no homologs for cps19fI and -J. In this study we used long-range PCR to amplify and clone a 10.5-kb section of the S. pneumoniae type 19B capsule locus (cps19b) between cps19bH and cps19bK. This region of the cps19b locus is 4 kb larger than that in the cps19f locus and replaces cps19fI and cps19fJ with five new ORFs, designated cps19bP, -I, -Q, -R, and -J. We have proposed functions for four of the protein products, including functional homologs of Cps19fI and Cps19fJ. Transformation of a S. pneumoniae mutant containing an interrupted type 19F capsule locus with the 10.5-kb cps19b PCR product converted the recipient strain to type 19B. Southern hybridization analysis indicated that cps19bP, -I, -Q, -R, and -J are unique to type 19B and the closely related type 19C. |
Keywords: | Streptococcus pneumoniae Bacterial Capsules Chromosome Mapping Transformation, Bacterial Amino Acid Sequence Base Sequence Sequence Homology, Amino Acid Genes, Bacterial Molecular Sequence Data |
DOI: | 10.1128/jb.179.15.4953-4958.1997 |
Published version: | http://dx.doi.org/10.1128/jb.179.15.4953-4958.1997 |
Appears in Collections: | Aurora harvest 2 Microbiology and Immunology publications |
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