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Type: Journal article
Title: Real-time binding monitoring between human blood proteins and heavy metal ions in nanoporous anodic alumina photonic crystals
Author: Law, C.
Lim, S.
Abell, A.
Santos, A.
Citation: Analytical Chemistry, 2018; 90(16):10039-10048
Publisher: American Chemical Society
Issue Date: 2018
ISSN: 0003-2700
Statement of
Cheryl Suwen Law, Siew Yee Lim, Andrew D. Abell, and Abel Santos
Abstract: This study reports on the real-time binding assessment between heavy metal ions and blood proteins immobilized onto nanoporous anodic alumina photonic crystals (NAA-PCs) by reflectometric interference spectroscopy (RIfS). The surface of NAA-PCs is chemically functionalized with γ-globulin (GG), transferrin (TFN), and serum albumin (HSA), the major proteins present in human blood plasma. Protein-modified NAA-PC platforms are exposed to analytical solutions of mercury ions of different concentrations. Dynamic changes in the effective optical thickness of protein-modified NAA-PCs in response to heavy metal ions are assessed in real time to evaluate the binding kinetics, affinity, and mechanism. Protein molecules undergo conformational changes upon exposure to mercury ions, with HSA exhibiting the strongest affinity. The combination of protein-modified NAA-PCs with RIfS allows real-time monitoring of protein-heavy metal ions interactions under dynamic flow conditions. This system is capable of detecting dynamic conformational changes in these proteins upon exposure to heavy metal ions. Our results provide new insights into these binding events, which could enable new methodologies to study the toxicity of heavy metal ions and other biomolecular interactions.
Keywords: Humans
Aluminum Oxide
Metals, Heavy
Spectrum Analysis
Protein Conformation
Protein Binding
Serum Albumin, Human
Rights: © 2018 American Chemical Society
DOI: 10.1021/acs.analchem.8b02732
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